Levels of the E2 interacting protein TopBP1 modulate papillomavirus maintenance stage replication
dc.contributor.author | Kanginakudru, Sriramana | |
dc.contributor.author | DeSmet, Marsha | |
dc.contributor.author | Thomas, Yanique | |
dc.contributor.author | Morgan, Iain M. | |
dc.contributor.author | Androphy, Elliot J. | |
dc.contributor.department | Department of Dermatology, IU School of Medicine | en_US |
dc.date.accessioned | 2016-08-05T16:34:17Z | |
dc.date.available | 2016-08-05T16:34:17Z | |
dc.date.issued | 2015-04 | |
dc.description.abstract | The evolutionarily conserved DNA topoisomerase II beta-binding protein 1 (TopBP1) functions in DNA replication, DNA damage response, and cell survival. We analyzed the role of TopBP1 in human and bovine papillomavirus genome replication. Consistent with prior reports, TopBP1 co-localized in discrete nuclear foci and was in complex with papillomavirus E2 protein. Similar to E2, TopBP1 is recruited to the region of the viral origin of replication during G1/S and early S phase. TopBP1 knockdown increased, while over-expression decreased transient virus replication, without affecting cell cycle. Similarly, using cell lines harboring HPV-16 or HPV-31 genome, TopBP1 knockdown increased while over-expression reduced viral copy number relative to genomic DNA. We propose a model in which TopBP1 serves dual roles in viral replication: it is essential for initiation of replication yet it restricts viral copy number. | en_US |
dc.eprint.version | Author's manuscript | en_US |
dc.identifier.citation | Kanginakudru, S., DeSmet, M., Thomas, Y., Morgan, I. M., & Androphy, E. J. (2015). Levels of the E2 interacting protein TopBP1 modulate papillomavirus maintenance stage replication. Virology, 478, 135–142. http://doi.org/10.1016/j.virol.2015.01.011 | en_US |
dc.identifier.issn | 1096-0341 | en_US |
dc.identifier.uri | https://hdl.handle.net/1805/10583 | |
dc.language.iso | en_US | en_US |
dc.publisher | Elsevier | en_US |
dc.relation.isversionof | 10.1016/j.virol.2015.01.011 | en_US |
dc.relation.journal | Virology | en_US |
dc.rights | Publisher Policy | en_US |
dc.source | PMC | en_US |
dc.subject | Carrier Proteins | en_US |
dc.subject | metabolism | en_US |
dc.subject | DNA-Binding Proteins | en_US |
dc.subject | Host-Pathogen Interactions | en_US |
dc.subject | Oncogene Proteins, Viral | en_US |
dc.subject | Papillomaviridae | en_US |
dc.subject | physiology | en_US |
dc.subject | Viral Proteins | en_US |
dc.subject | Virus Replication | en_US |
dc.title | Levels of the E2 interacting protein TopBP1 modulate papillomavirus maintenance stage replication | en_US |
dc.type | Article | en_US |