Characterization of a 3rd Generation Lentiviral Vector Pseudotyped With Nipah Virus Envelope Proteins For Endothelial Cell Transduction

dc.contributor.authorWitting, Scott R.
dc.contributor.authorVallanda, Priya
dc.contributor.authorGamble, Aisha L.
dc.contributor.departmentMedical and Molecular Genetics, School of Medicine
dc.date.accessioned2025-05-08T12:15:57Z
dc.date.available2025-05-08T12:15:57Z
dc.date.issued2013
dc.description.abstractLentiviruses are becoming progressively more popular as gene therapy vectors due to their ability to integrate into quiescent cells and recent clinical trial successes. Directing these vectors to specific cell types and limiting off-target transduction in vivo remains a challenge. Replacing the viral envelope proteins responsible for cellular binding, or pseudotyping, remains a common method to improve lentiviral targeting. Here, we describe the development of a high titer, third generation lentiviral vector pseudotyped with Nipah virus fusion protein (NiV-F) and attachment protein (NiV-G). Critical to high titers was truncation of the cytoplasmic domains of both NiV-F and NiV-G. As known targets of wild-type Nipah virus, primary endothelial cells are shown to be effectively transduced by the Nipah pseudotype. In contrast, human CD34+ hematopoietic progenitors were not significantly transduced. Additionally, the Nipah pseudotype has increased stability in human serum compared with vesicular stomatitis virus pseudotyped lentivirus. These findings suggest that the use of Nipah virus envelope proteins in third generation lentiviral vectors would be a valuable tool for gene delivery targeted to endothelial cells.
dc.eprint.versionAuthor's manuscript
dc.identifier.citationWitting SR, Vallanda P, Gamble AL. Characterization of a third generation lentiviral vector pseudotyped with Nipah virus envelope proteins for endothelial cell transduction. Gene Ther. 2013;20(10):997-1005. doi:10.1038/gt.2013.23
dc.identifier.urihttps://hdl.handle.net/1805/47885
dc.language.isoen_US
dc.publisherSpringer Nature
dc.relation.isversionof10.1038/gt.2013.23
dc.relation.journalGene Therapy
dc.rightsPublisher Policy
dc.sourcePMC
dc.subjectLentivirus
dc.subjectPseudotype
dc.subjectEndothelial cell
dc.subjectNipah virus
dc.titleCharacterization of a 3rd Generation Lentiviral Vector Pseudotyped With Nipah Virus Envelope Proteins For Endothelial Cell Transduction
dc.typeArticle
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