Protocol to evaluate fasting metabolism and its relationship to the core circadian clock in mice
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2025
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American English
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Elsevier
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Abstract
To investigate the role of hepatocyte genes in fasting metabolism, it is essential to analyze their expression across the entire 24-h circadian cycle. Here, we present a protocol to evaluate fasting metabolism and its relationship to the core circadian clock in mice. We describe steps for time-course fasting/feeding experiment setup and performing staggered fasting and refeeding for 24 h. We then detail procedures for evaluating oxidative substrate selection and rescuing defective oxidative metabolism through FGF21 and CPI-613 injections. For complete details on the use and execution of this protocol, please refer to Sun et al.
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Sun J, DeBosch BJ. Protocol to evaluate fasting metabolism and its relationship to the core circadian clock in mice. STAR Protoc. 2025;6(1):103660. doi:10.1016/j.xpro.2025.103660
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STAR Protocols
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Article
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Final published version