Decrease in Numbers of Naive and Resting B Cells in HIV-Infected Kenyan Adults Leads to a Proportional Increase in Total and Plasmodium falciparum-Specific Atypical Memory B Cells
dc.contributor.author | Frosch, Anne E. | |
dc.contributor.author | Odumade, Oludare A. | |
dc.contributor.author | Taylor, Justin J. | |
dc.contributor.author | Ireland, Kathleen | |
dc.contributor.author | Ayodo, George | |
dc.contributor.author | Ondigo, Bartholomew | |
dc.contributor.author | Narum, David L. | |
dc.contributor.author | Vulule, John | |
dc.contributor.author | John, Chandy C. | |
dc.contributor.department | Medicine, School of Medicine | en_US |
dc.date.accessioned | 2017-12-21T15:56:18Z | |
dc.date.available | 2017-12-21T15:56:18Z | |
dc.date.issued | 2017-06-15 | |
dc.description.abstract | Human immunodeficiency virus type 1 (HIV-1) infection is associated with B cell activation and exhaustion, and hypergammaglobulinemia. How these changes influence B cell responses to coinfections such as malaria is poorly understood. To address this, we compared B cell phenotypes and Abs specific for the Plasmodium falciparum vaccine candidate apical membrane Ag-1 (AMA1) in HIV-infected and uninfected adults living in Kenya. Surprisingly, HIV-1 infection was not associated with a difference in serum AMA1-specific Ab levels. HIV-infected individuals had a higher proportion of total atypical and total activated memory B cells (MBCs). Using an AMA1 tetramer to detect AMA1-specific B cells, HIV-infected individuals were also shown to have a higher proportion of AMA1-specific atypical MBCs. However, this proportional increase resulted in large part from a loss in the number of naive and resting MBCs rather than an increase in the number of atypical and activated cells. The loss of resting MBCs and naive B cells was mirrored in a population of cells specific for an Ag to which these individuals were unlikely to have been chronically exposed. Together, the data show that changes in P. falciparum Ag-specific B cell subsets in HIV-infected individuals mirror those in the overall B cell population, and suggest that the increased proportion of atypical MBC phenotypes found in HIV-1-infected individuals results from the loss of naive and resting MBCs. | en_US |
dc.eprint.version | Final published version | en_US |
dc.identifier.citation | Frosch, A. E., Odumade, O. A., Taylor, J. J., Ireland, K., Ayodo, G., Ondigo, B., … John, C. C. (2017). Decrease in Numbers of Naive and Resting B Cells in HIV-Infected Kenyan Adults Leads to a Proportional Increase in Total and Plasmodium falciparum–Specific Atypical Memory B Cells. The Journal of Immunology Author Choice, 198(12), 4629–4638. http://doi.org/10.4049/jimmunol.1600773 | en_US |
dc.identifier.uri | https://hdl.handle.net/1805/14859 | |
dc.language.iso | en_US | en_US |
dc.publisher | American Association of Immunologists | en_US |
dc.relation.isversionof | 10.4049/jimmunol.1600773 | en_US |
dc.relation.journal | The Journal of Immunology Author Choice | en_US |
dc.rights | Publisher Policy | en_US |
dc.source | PMC | en_US |
dc.subject | HIV infections | en_US |
dc.subject | Flow cytometry | en_US |
dc.subject | Immunologic memory | en_US |
dc.subject | Immunophenotyping | en_US |
dc.subject | Lymphocyte activation | en_US |
dc.title | Decrease in Numbers of Naive and Resting B Cells in HIV-Infected Kenyan Adults Leads to a Proportional Increase in Total and Plasmodium falciparum-Specific Atypical Memory B Cells | en_US |
dc.type | Article | en_US |
ul.alternative.fulltext | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5458331/ | en_US |