Characterization of Vaginal Microbial Community Dynamics in the Pathogenesis of Incident Bacterial Vaginosis, a Pilot Study

dc.contributor.authorElnaggar, Jacob H.
dc.contributor.authorLammons, John W.
dc.contributor.authorTaylor, Christopher M.
dc.contributor.authorToh, Evelyn
dc.contributor.authorArdizzone, Caleb M.
dc.contributor.authorDong, Amy
dc.contributor.authorAaron, Kristal J.
dc.contributor.authorLuo, Meng
dc.contributor.authorTamhane, Ashutosh
dc.contributor.authorLefkowitz, Elliot J.
dc.contributor.authorQuayle, Alison J.
dc.contributor.authorNelson, David E.
dc.contributor.authorMuzny, Christina A.
dc.contributor.departmentMicrobiology and Immunology, School of Medicine
dc.date.accessioned2024-10-15T11:42:36Z
dc.date.available2024-10-15T11:42:36Z
dc.date.issued2023
dc.description.abstractBackground: Despite more than 60 years of research, the etiology of bacterial vaginosis (BV) remains controversial. In this pilot study, we used shotgun metagenomic sequencing to characterize vaginal microbial community changes before the development of incident BV (iBV). Methods: A cohort of African American women with a baseline healthy vaginal microbiome (no Amsel criteria, Nugent score 0-3 with no Gardnerella vaginalis morphotypes) were followed for 90 days with daily self-collected vaginal specimens for iBV (≥2 consecutive days of a Nugent score of 7-10). Shotgun metagenomic sequencing was performed on select vaginal specimens from 4 women, every other day for 12 days before iBV diagnosis. Sequencing data were analyzed through Kraken2 and bioBakery 3 workflows, and specimens were classified into community state types. Quantitative polymerase chain reaction was performed to compare the correlation of read counts with bacterial abundance. Results: Common BV-associated bacteria such as G. vaginalis , Prevotella bivia , and Fannyhessea vaginae were increasingly identified in the participants before iBV. Linear modeling indicated significant increases in G. vaginalis and F . vaginae relative abundance before iBV, whereas the relative abundance of Lactobacillus species declined over time. The Lactobacillus species decline correlated with the presence of Lactobacillus phages. We observed enrichment in bacterial adhesion factor genes on days before iBV. There were also significant correlations between bacterial read counts and abundances measured by quantitative polymerase chain reaction. Conclusions: This pilot study characterizes vaginal community dynamics before iBV and identifies key bacterial taxa and mechanisms potentially involved in the pathogenesis of iBV.
dc.eprint.versionAuthor's manuscript
dc.identifier.citationElnaggar JH, Lammons JW, Taylor CM, et al. Characterization of Vaginal Microbial Community Dynamics in the Pathogenesis of Incident Bacterial Vaginosis, a Pilot Study. Sex Transm Dis. 2023;50(8):523-530. doi:10.1097/OLQ.0000000000001821
dc.identifier.urihttps://hdl.handle.net/1805/43959
dc.language.isoen_US
dc.publisherWolters Kluwer
dc.relation.isversionof10.1097/OLQ.0000000000001821
dc.relation.journalSexually Transmitted Diseases
dc.rightsPublisher Policy
dc.sourcePMC
dc.subjectBacteria
dc.subjectGardnerella vaginalis
dc.subjectLactobacillus
dc.subjectMicrobiota
dc.subjectVagina
dc.titleCharacterization of Vaginal Microbial Community Dynamics in the Pathogenesis of Incident Bacterial Vaginosis, a Pilot Study
dc.typeArticle
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Elnaggar2023Characterization-AAM.pdf
Size:
1.22 MB
Format:
Adobe Portable Document Format
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.04 KB
Format:
Item-specific license agreed upon to submission
Description: