60kDa Lysophospholipase, a New Sgk1 Molecular Partner Involved in the Regulation of ENaC
dc.contributor.author | Menniti, Miranda | |
dc.contributor.author | Iuliano, Rodolfo | |
dc.contributor.author | Föller, Michael | |
dc.contributor.author | Sopjani, Mentor | |
dc.contributor.author | Alesutan, Ioana | |
dc.contributor.author | Mariggiò, Stefania | |
dc.contributor.author | Nofziger, Charity | |
dc.contributor.author | Perri, Angela M. | |
dc.contributor.author | Amato, Rosario | |
dc.contributor.author | Blazer-Yost, Bonnie | |
dc.contributor.author | Corda, Daniela | |
dc.contributor.author | Lang, Florian | |
dc.contributor.author | Perrotti, Nicola | |
dc.date.accessioned | 2018-08-15T18:55:58Z | |
dc.date.available | 2018-08-15T18:55:58Z | |
dc.date.issued | 2010 | |
dc.description.abstract | The serum- and glucocorticoid-regulated kinase (Sgk1) is essential for hormonal regulation of ENaC-mediated sodium transport and is involved in the transduction of growth-factor-dependent cell survival and proliferation. The identification of molecular partners for Sgk1 is crucial for the understanding of its mechanisms of action. We performed a yeast two-hybrid screening based on a human kidney cDNA library to identify molecular partners of Sgk1. As a result the screening revealed a specific interaction between Sgk1 and a 60 kDa Lysophospholipase (LysoLP). LysoLP is a poorly characterized enzyme that, based on sequence analysis, might possess lysophospholipase and asparaginase activities. We demonstrate that LysoLP has indeed a lysophospholipase activity and affects metabolic functions related to cell proliferation and regulation of membrane channels. Moreover we demonstrate in the Xenopus oocyte expression system that LysoLP downregulates basal and Sgk1-dependent ENaC activity. In conclusion LysoLP may represent a new player in the regulation of ENaC and Sgk1-dependent signaling. | en_US |
dc.identifier.citation | Miranda Menniti, Rodolfo Iuliano, Michael Föller, Mentor Sopjani, Ioana Alesutan, Stefania Mariggiò, Charity Nofziger, Angela M. Perri, Rosario Amato, Bonnie Blazer-Yost, Daniela Corda, Florian Lang, and Nicola Perrotti. 60kDa Lysophospholipase, a New Sgk1 Molecular Partner Involved in the Regulation of ENaC. Cellular Physiology and Biochemistry. (2010). | en_US |
dc.identifier.uri | https://hdl.handle.net/1805/17146 | |
dc.subject | Epithelial Sodium Channels | en_US |
dc.subject | Immediate-Early Proteins | en_US |
dc.subject | Lysophospholipase | en_US |
dc.subject | Protein-Serine-Threonine Kinases | en_US |
dc.title | 60kDa Lysophospholipase, a New Sgk1 Molecular Partner Involved in the Regulation of ENaC | en_US |
dc.type | Article | en_US |