Low-Temperature Plasma Short Exposure to Decontaminate Peri-Implantitis-Related Multispecies Biofilms on Titanium Surfaces In Vitro

dc.contributor.authorPanariello, Beatriz H. D.
dc.contributor.authorMody, Drashty P.
dc.contributor.authorEckert, George J.
dc.contributor.authorWitek, Lukasz
dc.contributor.authorCoelho, Paulo G.
dc.contributor.authorDuarte, Simone
dc.contributor.departmentBiostatistics and Health Data Science, School of Medicine
dc.date.accessioned2024-01-10T13:16:28Z
dc.date.available2024-01-10T13:16:28Z
dc.date.issued2022-10-26
dc.description.abstractBackground: The use of low-temperature plasma (LTP) is a novel approach to treating peri-implantitis. LTP disrupts the biofilm while conditioning the surrounding host environment for bone growth around the infected implant. The main objective of this study was to evaluate the antimicrobial properties of LTP on newly formed (24 h), intermediate (3 days), and mature (7 days) peri-implant-related biofilms formed on titanium surfaces. Methods: Actinomyces naeslundii (ATCC 12104), Porphyromonas gingivalis (W83), Streptococcus oralis (ATCC 35037), and Veillonella dispar (ATCC 17748) were cultivated in brain heart infusion supplemented with 1% yeast extract, hemin (0.5 mg/mL), and menadione (5 mg/mL) and kept at 37°C in anaerobic conditions for 24 h. Species were mixed for a final concentration of ~105 colony forming units (CFU)/mL (OD = 0.01), and the bacterial suspension was put in contact with titanium specimens (7.5 mm in diameter by 2 mm in thickness) for biofilm formation. Biofilms were treated with LTP for 1, 3, and 5 min at 3 or 10 mm from plasma tip to sample. Controls were those having no treatment (negative control, NC) and argon flow under the same LTP conditions. Positive controls were those treated with 14 μg/mL amoxicillin and 140 μg/mL metronidazole individually or combined and 0.12% chlorhexidine (n = 6 per group). Biofilms were evaluated by CFU, confocal laser scanning microscopy (CLSM), and fluorescence in situ hybridization (FISH). Comparisons among bacteria; 24 h, 3-day, and 7-day biofilms; and treatments for each biofilm were made. Wilcoxon signed-rank and Wilcoxon rank sum tests were applied (α = 0.05). Results: Bacterial growth was observed in all NC groups, corroborated by FISH. LTP treatment significantly reduced all bacteria species compared to the NC in all biofilm periods and treatment conditions (p ≤ 0.016), and CLSM corroborated these results. Conclusion: Within the limitation of this study, we conclude that LTP application effectively reduces peri-implantitis-related multispecies biofilms on titanium surfaces in vitro.
dc.eprint.versionFinal published version
dc.identifier.citationPanariello BHD, Mody DP, Eckert GJ, Witek L, Coelho PG, Duarte S. Low-Temperature Plasma Short Exposure to Decontaminate Peri-Implantitis-Related Multispecies Biofilms on Titanium Surfaces In Vitro. Biomed Res Int. 2022;2022:1549774. Published 2022 Oct 26. doi:10.1155/2022/1549774
dc.identifier.urihttps://hdl.handle.net/1805/37924
dc.language.isoen_US
dc.publisherHindawi
dc.relation.isversionof10.1155/2022/1549774
dc.relation.journalBioMed Research International
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourcePMC
dc.subjectBacteria
dc.subjectBiofilms
dc.subjectPeri-Implantitis
dc.subjectTemperature
dc.subjectTitanium
dc.titleLow-Temperature Plasma Short Exposure to Decontaminate Peri-Implantitis-Related Multispecies Biofilms on Titanium Surfaces In Vitro
dc.typeArticle
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
BMRI2022-1549774.pdf
Size:
5.07 MB
Format:
Adobe Portable Document Format
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.99 KB
Format:
Item-specific license agreed upon to submission
Description: