Regulation of Constitutive Tip110 Expression in Human Cord Blood CD34+ Cells Through Selective Usage of the Proximal and Distal Polyadenylation Sites Within the 3′Untranslated Region

dc.contributor.authorLiu, Ying
dc.contributor.authorHuang, Xinxin
dc.contributor.authorTimani, Khalid A.
dc.contributor.authorBroxmeyer, Hal E.
dc.contributor.authorHe, Johnny J.
dc.contributor.departmentMicrobiology and Immunology, School of Medicineen_US
dc.date.accessioned2019-03-08T19:28:42Z
dc.date.available2019-03-08T19:28:42Z
dc.date.issued2018
dc.description.abstractTip110 plays important roles for stem cell pluripotency and hematopoiesis. However, little is known about the regulatory mechanisms of Tip110 expression in this process. In this study, we first showed that constitutive Tip110 expression was cell proliferation and differentiation dependent and self-regulated in both human cord blood CD34+ cells. Using a series of molecular techniques, we found that ectopic Tip110 expression led to increased constitutive Tip110 expression through its 3′-untranslated region (3′UTR), specifically through preferential usage of proximal polyadenylation sites within its 3′UTR in cells, including human cord blood CD34+ cells, which indeed led to an increased number of CD34+ cells during differentiation of those cells. Lastly, we showed that Tip110 protein interacted with cleavage stimulation factor 64 (CstF64) protein and that more CstF64 was recruited to the promixal polyadenylation site than the distal polyadenylation site within its 3′UTR. These finding together demonstrates that constitutive Tip110 expression is regulated, at least in part, through its interaction with CstF64, recruitment of CstF64 to, and selective usage of those two polyadenylation sites within its 3′UTR.en_US
dc.eprint.versionFinal published versionen_US
dc.identifier.citationLiu, Y., Huang, X., Timani, K. A., Broxmeyer, H. E., & He, J. J. (2018). Regulation of Constitutive Tip110 Expression in Human Cord Blood CD34+ Cells Through Selective Usage of the Proximal and Distal Polyadenylation Sites Within the 3′Untranslated Region. Stem Cells and Development, 27(8), 566–576. https://doi.org/10.1089/scd.2017.0197en_US
dc.identifier.urihttps://hdl.handle.net/1805/18584
dc.language.isoenen_US
dc.publisherLieberten_US
dc.relation.isversionof10.1089/scd.2017.0197en_US
dc.relation.journalStem Cells and Developmenten_US
dc.rightsPublisher Policyen_US
dc.sourceAuthoren_US
dc.subjecthematopoiesisen_US
dc.subjectCD34+en_US
dc.subjectmiRNAen_US
dc.titleRegulation of Constitutive Tip110 Expression in Human Cord Blood CD34+ Cells Through Selective Usage of the Proximal and Distal Polyadenylation Sites Within the 3′Untranslated Regionen_US
dc.typeArticleen_US
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