Direction Selectivity in Drosophila Proprioceptors Requires the Mechanosensory Channel Tmc

dc.contributor.authorHe, Liping
dc.contributor.authorGulyanon, Sarun
dc.contributor.authorMihovilovic Skanata, Mirna
dc.contributor.authorKaragyozov, Doycho
dc.contributor.authorHeckscher, Ellie S.
dc.contributor.authorKrieg, Michael
dc.contributor.authorTsechpenakis, Gavriil
dc.contributor.authorGershow, Marc
dc.contributor.authorTracey, W. Daniel
dc.contributor.departmentDepartment of Computer and Information sciences, School of Scienceen_US
dc.date.accessioned2019-09-20T18:05:39Z
dc.date.available2019-09-20T18:05:39Z
dc.date.issued2019-03-18
dc.description.abstractSummary Drosophila Transmembrane channel-like (Tmc) is a protein that functions in larval proprioception. The closely related TMC1 protein is required for mammalian hearing and is a pore-forming subunit of the hair cell mechanotransduction channel. In hair cells, TMC1 is gated by small deflections of microvilli that produce tension on extracellular tip-links that connect adjacent villi. How Tmc might be gated in larval proprioceptors, which are neurons having a morphology that is completely distinct from hair cells, is unknown. Here, we have used high-speed confocal microscopy both to measure displacements of proprioceptive sensory dendrites during larval movement and to optically measure neural activity of the moving proprioceptors. Unexpectedly, the pattern of dendrite deformation for distinct neurons was unique and differed depending on the direction of locomotion: ddaE neuron dendrites were strongly curved by forward locomotion, while the dendrites of ddaD were more strongly deformed by backward locomotion. Furthermore, GCaMP6f calcium signals recorded in the proprioceptive neurons during locomotion indicated tuning to the direction of movement. ddaE showed strong activation during forward locomotion, while ddaD showed responses that were strongest during backward locomotion. Peripheral proprioceptive neurons in animals mutant for Tmc showed a near-complete loss of movement related calcium signals. As the strength of the responses of wild-type animals was correlated with dendrite curvature, we propose that Tmc channels may be activated by membrane curvature in dendrites that are exposed to strain. Our findings begin to explain how distinct cellular systems rely on a common molecular pathway for mechanosensory responses.en_US
dc.eprint.versionFinal published versionen_US
dc.identifier.citationHe, L., Gulyanon, S., Mihovilovic Skanata, M., Karagyozov, D., Heckscher, E. S., Krieg, M., … Tracey, W. D. (2019). Direction Selectivity in Drosophila Proprioceptors Requires the Mechanosensory Channel Tmc. Current Biology, 29(6), 945-956.e3. https://doi.org/10.1016/j.cub.2019.02.025en_US
dc.identifier.issn0960-9822en_US
dc.identifier.urihttps://hdl.handle.net/1805/20965
dc.language.isoen_USen_US
dc.publisherElsevieren_US
dc.relation.isversionof10.1016/j.cub.2019.02.025en_US
dc.relation.journalCurrent Biologyen_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.sourcePublisheren_US
dc.subjectproprioceptionen_US
dc.subjectDrosophila melanogasteren_US
dc.subjectmechanotransductionen_US
dc.subjectmicroscopyen_US
dc.subjectTmcen_US
dc.subjectsensoryen_US
dc.subjectbehavioren_US
dc.titleDirection Selectivity in Drosophila Proprioceptors Requires the Mechanosensory Channel Tmcen_US
dc.typeArticleen_US
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