Generating Inner Ear Organoids from Mouse Embryonic Stem Cells
dc.contributor.author | Longworth-Mills, Emma | |
dc.contributor.author | Koehler, Karl R. | |
dc.contributor.author | Hashino, Eri | |
dc.contributor.department | Otolaryngology -- Head and Neck Surgery, School of Medicine | en_US |
dc.date.accessioned | 2019-07-26T14:29:43Z | |
dc.date.available | 2019-07-26T14:29:43Z | |
dc.date.issued | 2016 | |
dc.description.abstract | This protocol describes a three-dimensional culture method for generating inner ear sensory epithelia, which comprises sensory hair cells and a concurrently arising neuronal population. Mouse embryonic stem cells are initially plated in 96-well plates with differentiation media; following aggregation, Matrigel is added in order to promote epithelialization. A series of small molecule applications is then used over the first 14 days of culture to guide differentiation towards an otic lineage. After 16-20 days, vesicles containing inner ear sensory hair cells and supporting cells arise from the cultured aggregates. Aggregates may be analyzed using immunohistochemistry and electrophysiology techniques. This system serves as a simple and relatively inexpensive in vitro model of inner ear development. | en_US |
dc.eprint.version | Author's manuscript | en_US |
dc.identifier.citation | Longworth-Mills, E., Koehler, K. R., & Hashino, E. (2016). Generating Inner Ear Organoids from Mouse Embryonic Stem Cells. Methods in molecular biology (Clifton, N.J.), 1341, 391–406. doi:10.1007/7651_2015_215 | en_US |
dc.identifier.uri | https://hdl.handle.net/1805/19965 | |
dc.language.iso | en_US | en_US |
dc.publisher | Springer | en_US |
dc.relation.isversionof | 10.1007/7651_2015_215 | en_US |
dc.relation.journal | Methods in Molecular Biology | en_US |
dc.rights | Publisher Policy | en_US |
dc.source | PMC | en_US |
dc.subject | Cell culture techniques | en_US |
dc.subject | Cell differentiation | en_US |
dc.subject | Hair cell | en_US |
dc.subject | Inner ear | en_US |
dc.subject | Neurogenesis | en_US |
dc.subject | Stem cell | en_US |
dc.subject | Three-dimensional cell culture | en_US |
dc.subject | Vestibular | en_US |
dc.title | Generating Inner Ear Organoids from Mouse Embryonic Stem Cells | en_US |
dc.type | Article | en_US |