Forming a Complex with MHC Class I Molecules Interferes with Mouse CD1d Functional Expression

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2013-08-29
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American English
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Abstract

CD1d molecules are structurally similar to MHC class I, but present lipid antigens as opposed to peptides. Here, we show that MHC class I molecules physically associate with (and regulate the functional expression of) mouse CD1d on the surface of cells. Low pH (3.0) acid stripping of MHC class I molecules resulted in increased surface expression of murine CD1d on antigen presenting cells as well as augmented CD1d-mediated antigen presentation to NKT cells. Consistent with the above results, TAP1-/- mice were found to have a higher percentage of type I NKT cells as compared to wild type mice. Moreover, bone marrow-derived dendritic cells from TAP1-/- mice showed increased antigen presentation by CD1d compared to wild type mice. Together, these results suggest that MHC class I molecules can regulate NKT cell function, in part, by masking CD1d.

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Cite As
Gourapura RJ, Khan MA, Gallo RM, Shaji D, Liu J, Brutkiewicz RR. Forming a complex with MHC class I molecules interferes with mouse CD1d functional expression. PLoS One. 2013;8(8):e72867. Published 2013 Aug 29. doi:10.1371/journal.pone.0072867
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PLoS One
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PMC
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