Expanded LUXendin Color Palette for GLP1R Detection and Visualization In Vitro and In Vivo

dc.contributor.authorAst, Julia
dc.contributor.authorNovak, Alissa N.
dc.contributor.authorPodewin, Tom
dc.contributor.authorFine, Nicholas H.F.
dc.contributor.authorJones, Ben
dc.contributor.authorTomas, Alejandra
dc.contributor.authorBirke, Ramona
dc.contributor.authorRoßmann, Kilian
dc.contributor.authorEichhorst, Jenny
dc.contributor.authorLehmann, Martin
dc.contributor.authorLinnemann, Amelia K.
dc.contributor.authorHodson, David J.
dc.contributor.authorBroichhagen, Johannes
dc.contributor.departmentPediatrics, School of Medicineen_US
dc.date.accessioned2023-06-16T17:53:55Z
dc.date.available2023-06-16T17:53:55Z
dc.date.issued2022-04-04
dc.description.abstractThe glucagon-like peptide-1 receptor (GLP1R) is expressed in peripheral tissues and the brain, where it exerts pleiotropic actions on metabolic and inflammatory processes. Detection and visualization of GLP1R remains challenging, partly due to a lack of validated reagents. Previously, we generated LUXendins, antagonistic red and far-red fluorescent probes for specific labeling of GLP1R in live and fixed cells/tissues. We now extend this concept to the green and near-infrared color ranges by synthesizing and testing LUXendin492, LUXendin551, LUXendin615, and LUXendin762. All four probes brightly and specifically label GLP1R in cells and pancreatic islets. Further, LUXendin551 acts as a chemical beta cell reporter in preclinical rodent models, while LUXendin762 allows noninvasive imaging, highlighting differentially accessible GLP1R populations. We thus expand the color palette of LUXendins to seven different spectra, opening up a range of experiments using wide-field microscopy available in most labs through super-resolution imaging and whole animal imaging. With this, we expect that LUXendins will continue to generate novel and specific insights into GLP1R biology.en_US
dc.eprint.versionFinal published versionen_US
dc.identifier.citationAst J, Novak AN, Podewin T, et al. Expanded LUXendin Color Palette for GLP1R Detection and Visualization In Vitro and In Vivo. JACS Au. 2022;2(4):1007-1017. Published 2022 Apr 4. doi:10.1021/jacsau.2c00130en_US
dc.identifier.urihttps://hdl.handle.net/1805/33825
dc.language.isoen_USen_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.isversionof10.1021/jacsau.2c00130en_US
dc.relation.journalJACS Auen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0*
dc.sourcePMCen_US
dc.subjectIncretinen_US
dc.subjectGLP1Ren_US
dc.subjectDiabetesen_US
dc.subjectBeta cellen_US
dc.subjectFluorescent probesen_US
dc.subjectNoninvasive imagingen_US
dc.titleExpanded LUXendin Color Palette for GLP1R Detection and Visualization In Vitro and In Vivoen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
au2c00130.pdf
Size:
13.8 MB
Format:
Adobe Portable Document Format
Description:
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.99 KB
Format:
Item-specific license agreed upon to submission
Description: