Fluorescent proteins for FRET microscopy: monitoring protein interactions in living cells

dc.contributor.authorDay, Richard N.
dc.contributor.authorDavidson, Michael W.
dc.contributor.departmentCellular and Integrative Physiology, School of Medicine
dc.date.accessioned2025-07-09T19:02:03Z
dc.date.available2025-07-09T19:02:03Z
dc.date.issued2012
dc.description.abstractThe discovery and engineering of novel fluorescent proteins (FPs) from diverse organisms is yielding fluorophores with exceptional characteristics for live-cell imaging. In particular, the development of FPs for fluorescence (or Förster) resonance energy transfer (FRET) microscopy is providing important tools for monitoring dynamic protein interactions inside living cells. The increased interest in FRET microscopy has driven the development of many different methods to measure FRET. However, the interpretation of FRET measurements is complicated by several factors including the high fluorescence background, the potential for photoconversion artifacts and the relatively low dynamic range afforded by this technique. Here, we describe the advantages and disadvantages of four methods commonly used in FRET microscopy. We then discuss the selection of FPs for the different FRET methods, identifying the most useful FP candidates for FRET microscopy. The recent success in expanding the FP color palette offers the opportunity to explore new FRET pairs.
dc.eprint.versionAuthor's manuscript
dc.identifier.citationDay RN, Davidson MW. Fluorescent proteins for FRET microscopy: monitoring protein interactions in living cells [published correction appears in Bioessays. 2012 Jun;34(6):521]. Bioessays. 2012;34(5):341-350. doi:10.1002/bies.201100098
dc.identifier.urihttps://hdl.handle.net/1805/49301
dc.language.isoen_US
dc.publisherWiley
dc.relation.isversionof10.1002/bies.201100098
dc.relation.journalBioEssays
dc.rightsPublisher Policy
dc.sourcePMC
dc.subjectFluorescent protein
dc.subjectFluorescence resonance energy transfer (FRET)
dc.subjectFluorescence lifetime imaging microscopy (FLIM)
dc.subjectAcceptor photobleaching
dc.subjectSpectral imaging
dc.titleFluorescent proteins for FRET microscopy: monitoring protein interactions in living cells
dc.typeArticle
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Day2012Fluorescent-AAM.pdf
Size:
1.23 MB
Format:
Adobe Portable Document Format
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.04 KB
Format:
Item-specific license agreed upon to submission
Description: