Time-dependent stimulation by aldosterone of blocker-sensitive ENaCs in A6 epithelia
dc.contributor.author | Helman, Sandy I. | |
dc.contributor.author | Liu, Xuehong | |
dc.contributor.author | Baldwin, Kieron | |
dc.contributor.author | Blazer-Yost, Bonnie | |
dc.contributor.author | Els, Willem J. | |
dc.date.accessioned | 2018-08-09T19:47:23Z | |
dc.date.available | 2018-08-09T19:47:23Z | |
dc.date.issued | 1998-04 | |
dc.description.abstract | To study and define the early time-dependent response (< or = 6 h) of blocker-sensitive epithelial Na+ channels (ENaCs) to stimulation of Na+ transport by aldosterone, we used a new modified method of blocker-induced noise analysis to determine the changes of single-channel current (iNa) channel open probability (Po), and channel density (NT) under transient conditions of transport as measured by macroscopic short-circuit currents (Isc). In three groups of experiments in which spontaneous baseline rates of transport averaged 1.06, 5.40, and 15.14 microA/cm2, stimulation of transport occurred due to increase of blocker-sensitive channels. NT varied linearly over a 70-fold range of transport (0.5-35 microA/cm2). Relatively small and slow time-dependent but aldosterone-independent decreases of Po occurred during control (10-20% over 2 h) and aldosterone experimental periods (10-30% over 6 h). When the Po of control and aldosterone-treated tissues was examined over the 70-fold extended range of Na+ transport, Po was observed to vary inversely with Isc, falling from approximately 0.5 to approximately 0.15 at the highest rates of Na+ transport or approximately 25% per 3-fold increase of transport. Because decreases of Po from any source cannot explain stimulation of transport by aldosterone, it is concluded that the early time-dependent stimulation of Na+ transport in A6 epithelia is due exclusively to increase of apical membrane NT. | en_US |
dc.identifier.citation | Sandy I. Helman, Xuehong Liu, Kieron Baldwin, Bonnie Blazer-Yost, and Willem J. Els. Time-dependent stimulation by aldosterone of blocker-sensitive ENaCs in A6 epithelia. American Journal of Physiology-Cell Physiology. (1998). | en_US |
dc.identifier.uri | https://hdl.handle.net/1805/17063 | |
dc.subject | Aldosterone | en_US |
dc.subject | Sodium Channels | en_US |
dc.subject | Sodium Channels | en_US |
dc.title | Time-dependent stimulation by aldosterone of blocker-sensitive ENaCs in A6 epithelia | en_US |
dc.type | Article | en_US |