The Effects of GATA-1 and NF-E2 Deficiency on Bone Biomechanical, Biochemical, and Mineral Properties

dc.contributor.authorKacena, Melissa A.
dc.contributor.authorGundberg, Caren M.
dc.contributor.authorKacena, William J., III
dc.contributor.authorLandis, William J.
dc.contributor.authorBoskey, Adele L.
dc.contributor.authorBouxsein, Mary L.
dc.contributor.authorHorowitz, Mark C.
dc.contributor.departmentOrthopaedic Surgery, School of Medicine
dc.date.accessioned2025-05-27T13:07:20Z
dc.date.available2025-05-27T13:07:20Z
dc.date.issued2013
dc.description.abstractMice deficient in GATA-1 or NF-E2, transcription factors required for normal megakaryocyte (MK) development, have increased numbers of MKs, reduced numbers of platelets, and a striking high bone mass phenotype. Here, we show the bone geometry, microarchitecture, biomechanical, biochemical, and mineral properties from these mutant mice. We found that the outer geometry of the mutant bones was similar to controls, but that both mutants had a striking increase in total bone area (up to a 35% increase) and trabecular bone area (up to a 19% increase). Interestingly, only the NF-E2 deficient mice had a significant increase in cortical bone area (21%) and cortical thickness (27%), which is consistent with the increase in bone mineral density (BMD) seen only in the NF-E2 deficient femurs. Both mutant femurs exhibited significant increases in several biomechanical properties including peak load (up to a 32% increase) and stiffness (up to a 13% increase). Importantly, the data also demonstrate differences between the two mutant mice. GATA-1 deficient femurs break in a ductile manner, whereas NF-E2 deficient femurs are brittle in nature. To better understand these differences, we examined the mineral properties of these bones. Although none of the parameters measured were different between the NF-E2 deficient and control mice, an increase in calcium (21%) and an increase in the mineral/matrix ratio (32%) was observed in GATA-1 deficient mice. These findings appear to contradict biomechanical findings, suggesting the need for further research into the mechanisms by which GATA-1 and NF-E2 deficiency alter the material properties of bone.
dc.eprint.versionAuthor's manuscript
dc.identifier.citationKacena MA, Gundberg CM, Kacena WJ 3rd, et al. The effects of GATA-1 and NF-E2 deficiency on bone biomechanical, biochemical, and mineral properties. J Cell Physiol. 2013;228(7):1594-1600. doi:10.1002/jcp.24322
dc.identifier.urihttps://hdl.handle.net/1805/48389
dc.language.isoen_US
dc.publisherWiley
dc.relation.isversionof10.1002/jcp.24322
dc.relation.journalJournal of Cellular Physiology
dc.rightsPublisher Policy
dc.sourcePMC
dc.subjectFemur
dc.subjectBone density
dc.subjectMegakaryocytes
dc.subjectOsteoblasts
dc.titleThe Effects of GATA-1 and NF-E2 Deficiency on Bone Biomechanical, Biochemical, and Mineral Properties
dc.typeArticle
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Kacena2013Effects-AAM.pdf
Size:
256.93 KB
Format:
Adobe Portable Document Format
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.04 KB
Format:
Item-specific license agreed upon to submission
Description: