Measurement of mitochondrial respiration in the murine retina using a Seahorse extracellular flux analyzer
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Date
2021-06
Language
English
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Elsevier
Abstract
Mitochondrial metabolism is a critical mechanism that is deregulated in numerous retinal diseases. Here, we elaborate a protocol to quantify oxygen consumption rate as a measure of mitochondrial respiration directly from mouse retinal tissue pieces. Our procedure combines the use of Seahorse extracellular flux technology and ex vivo retinal tissue isolation and is robustly reproducible under different treatment conditions. This protocol allows direct assessment of mitochondrial function in response to drug treatments or genetic manipulation in mouse models.
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Shetty T, Park B, Corson TW. 2021. Measurement of mitochondrial respiration in murine retina using Seahorse extracellular flux analyzer. STAR Protocols, 2, 100533. https://doi.org/10.1016/j.xpro.2021.100533
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STAR Protocols
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