Extracellular matrix protein composition dynamically changes during murine forelimb development

dc.contributor.authorJacobson, Kathryn R.
dc.contributor.authorSaleh, Aya M.
dc.contributor.authorLipp, Sarah N.
dc.contributor.authorTian, Chengzhe
dc.contributor.authorWatson, Audrey R.
dc.contributor.authorLuetkemeyer, Callan M.
dc.contributor.authorOcken, Alexander R.
dc.contributor.authorSpencer, Sabrina L.
dc.contributor.authorKinzer-Ursem, Tamara L.
dc.contributor.authorCalve, Sarah
dc.contributor.departmentMedicine, School of Medicine
dc.date.accessioned2024-06-12T14:59:00Z
dc.date.available2024-06-12T14:59:00Z
dc.date.issued2024-01-09
dc.description.abstractThe extracellular matrix (ECM) is an integral part of multicellular organisms, connecting different cell layers and tissue types. During morphogenesis and growth, tissues undergo substantial reorganization. While it is intuitive that the ECM remodels in concert, little is known regarding how matrix composition and organization change during development. Here, we quantified ECM protein dynamics in the murine forelimb during appendicular musculoskeletal morphogenesis (embryonic days 11.5-14.5) using tissue fractionation, bioorthogonal non-canonical amino acid tagging, and mass spectrometry. Our analyses indicated that ECM protein (matrisome) composition in the embryonic forelimb changed as a function of development and growth, was distinct from other developing organs (brain), and was altered in a model of disease (osteogenesis imperfecta murine). Additionally, the tissue distribution for select matrisome was assessed via immunohistochemistry in the wild-type embryonic and postnatal musculoskeletal system. This resource will guide future research investigating the role of the matrisome during complex tissue development.
dc.eprint.versionFinal published version
dc.identifier.citationJacobson KR, Saleh AM, Lipp SN, et al. Extracellular matrix protein composition dynamically changes during murine forelimb development. iScience. 2024;27(2):108838. Published 2024 Jan 9. doi:10.1016/j.isci.2024.108838
dc.identifier.urihttps://hdl.handle.net/1805/41466
dc.language.isoen_US
dc.publisherElsevier
dc.relation.isversionof10.1016/j.isci.2024.108838
dc.relation.journaliScience
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourcePMC
dc.subjectBiological sciences
dc.subjectDevelopmental biology
dc.subjectNatural sciences
dc.titleExtracellular matrix protein composition dynamically changes during murine forelimb development
dc.typeArticle
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