Monitoring peripheral protein oligomerization on biological membranes

dc.contributor.authorStahelin, Robert V.
dc.contributor.departmentDepartment of Biochemistry & Molecular Biology, IU School of Medicineen_US
dc.date.accessioned2016-02-19T22:05:23Z
dc.date.available2016-02-19T22:05:23Z
dc.date.issued2013
dc.description.abstractPeripheral proteins transiently interact with cellular membranes where they regulate important cellular events such as signal transduction. A number of peripheral proteins harbor lipid-binding modules that not only bind selectively with nanomolar affinity to biological membranes but also oligomerize on the membrane surface. In some cases specific lipid binding or specific lipid compositions can induce peripheral protein oligomerization on cellular membranes. These oligomers serve different roles in biological signaling such as regulating protein-protein interactions, induction of membrane bending, or facilitating membrane scission. A number of technologies have been employed to study protein oligomerization with fluctuation analysis of fluorescently labeled molecules recently developed for use with commercial laser scanning microscopes. In this chapter the approach of Raster Image Correlation Spectroscopy coupled with Number and Brightness (N&B) analysis to investigate protein oligomerization on cellular membranes in live cells is presented. Important considerations are discussed for designing experiments, collecting data, and performing analysis. N&B analysis provides a robust method for assessing membrane binding and assembly properties of peripheral proteins and lipid-binding modules.en_US
dc.eprint.versionAuthor's manuscripten_US
dc.identifier.citationStahelin, R. V. (2013). Monitoring peripheral protein oligomerization on biological membranes. Methods in Cell Biology, 117, 359–371. http://doi.org/10.1016/B978-0-12-408143-7.00019-0en_US
dc.identifier.urihttps://hdl.handle.net/1805/8393
dc.language.isoen_USen_US
dc.publisherElsevier B.V.en_US
dc.relation.isversionof10.1016/B978-0-12-408143-7.00019-0en_US
dc.relation.journalMethods in Cell Biologyen_US
dc.rightsPublisher Policyen_US
dc.sourcePMCen_US
dc.subjectlipid bindingen_US
dc.subjectmembrane bindingen_US
dc.subjectoligomerizationen_US
dc.subjectperipheral proteinen_US
dc.subjectprotein assemblyen_US
dc.titleMonitoring peripheral protein oligomerization on biological membranesen_US
dc.typeArticleen_US
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