Bioinspired One Cell Culture Isolates Highly Tumorigenic and Metastatic Cancer Stem Cells Capable of Multilineage Differentiation

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dc.contributor.authorWang, Hai
dc.contributor.authorAgarwal, Pranay
dc.contributor.authorJiang, Bin
dc.contributor.authorStewart, Samantha
dc.contributor.authorLiu, Xuanyou
dc.contributor.authorLiang, Yutong
dc.contributor.authorHancioglu, Baris
dc.contributor.authorWebb, Amy
dc.contributor.authorFisher, John P.
dc.contributor.authorLiu, Zhenguo
dc.contributor.authorLu, Xiongbin
dc.contributor.authorTkaczuk, Katherine H. R.
dc.contributor.authorHe, Xiaoming
dc.contributor.departmentMedical and Molecular Genetics, School of Medicineen_US
dc.date.accessioned2020-10-08T13:52:17Z
dc.date.available2020-10-08T13:52:17Z
dc.date.issued2020-04-28
dc.description.sponsorshipCancer stem cells (CSCs) are rare cancer cells that are postulated to be responsible for cancer relapse and metastasis. However, CSCs are difficult to isolate and poorly understood. Here, a bioinspired approach for label‐free isolation and culture of CSCs, by microencapsulating one cancer cell in the nanoliter‐scale hydrogel core of each prehatching embryo‐like core–shell microcapsule, is reported. Only a small percentage of the individually microencapsulated cancer cells can proliferate into a cell colony. Gene and protein expression analyses indicate high stemness of the cells in the colonies. Importantly, the colony cells are capable of cross‐tissue multilineage (e.g., endothelial, cardiac, neural, and osteogenic) differentiation, which is not observed for “CSCs” isolated using other contemporary approaches. Further studies demonstrate the colony cells are highly tumorigenic, metastatic, and drug resistant. These data show the colony cells obtained with the bioinspired one‐cell‐culture approach are truly CSCs. Significantly, multiple pathways are identified to upregulate in the CSCs and enrichment of genes related to the pathways is correlated with significantly decreased survival of breast cancer patients. Collectively, this study may provide a valuable method for isolating and culturing CSCs, to facilitate the understanding of cancer biology and etiology and the development of effective CSC‐targeted cancer therapies.en_US
dc.eprint.versionFinal published versionen_US
dc.identifier.citationWang, H., Agarwal, P., Jiang, B., Stewart, S., Liu, X., Liang, Y., Hancioglu, B., Webb, A., Fisher, J. P., Liu, Z., Lu, X., Tkaczuk, K. H. R., & He, X. (2020). Bioinspired One Cell Culture Isolates Highly Tumorigenic and Metastatic Cancer Stem Cells Capable of Multilineage Differentiation. Advanced Science, 7(11), 2000259. https://doi.org/10.1002/advs.202000259en_US
dc.identifier.issn2198-3844en_US
dc.identifier.urihttps://hdl.handle.net/1805/24007
dc.language.isoen_USen_US
dc.publisherWileyen_US
dc.relation.isversionof10.1002/advs.202000259en_US
dc.relation.journalAdvanced Scienceen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.sourcePMCen_US
dc.subjectcancer stem cellsen_US
dc.subjectdifferentiation,en_US
dc.subjectdrug resistanceen_US
dc.subjectmetastasisen_US
dc.subjectmicrofluidicsen_US
dc.titleBioinspired One Cell Culture Isolates Highly Tumorigenic and Metastatic Cancer Stem Cells Capable of Multilineage Differentiationen_US
dc.typeArticleen_US
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