Targeted rescue of cancer-associated IDH1 mutant activity using an engineered synthetic antibody

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dc.contributor.authorRizk, Shahir S.
dc.contributor.authorMukherjee, Somnath
dc.contributor.authorKoide, Akiko
dc.contributor.authorKoide, Shohei
dc.contributor.authorKossiakoff, Anthony A.
dc.contributor.departmentDepartment of Biochemistry and Molecular Biology, School of Medicineen_US
dc.date.accessioned2017-08-24T17:55:08Z
dc.date.available2017-08-24T17:55:08Z
dc.date.issued2017-04-03
dc.description.abstractWe have utilized a high-diversity phage display library to engineer antibody fragments (Fabs) that can modulate the activity of the enzyme isocitrate dehydrogenase 1 (IDH1). We show that a conformation-specific Fab can reactivate an IDH1 mutant associated with brain tumors. The results show that this strategy is a first step towards developing "activator drugs" for a large number of genetic disorders where mutations have disrupted protein function.en_US
dc.identifier.citationRizk, S. S., Mukherjee, S., Koide, A., Koide, S., & Kossiakoff, A. A. (2017). Targeted rescue of cancer-associated IDH1 mutant activity using an engineered synthetic antibody. Scientific Reports, 7, 556. http://doi.org/10.1038/s41598-017-00728-1en_US
dc.identifier.urihttps://hdl.handle.net/1805/13909
dc.language.isoen_USen_US
dc.publisherSpringerNatureen_US
dc.relation.isversionof10.1038/s41598-017-00728-1en_US
dc.relation.journalScientific Reportsen_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/
dc.sourcePMCen_US
dc.subjectAntibody fragments (Fabs)en_US
dc.subjectEnzyme isocitrate dehydrogenase 1 (IDH1)en_US
dc.subjectIDH1 mutant associated with brain tumorsen_US
dc.subjectProtein functionen_US
dc.subjectMutationsen_US
dc.titleTargeted rescue of cancer-associated IDH1 mutant activity using an engineered synthetic antibodyen_US
dc.typeArticleen_US
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