Fecal bile acids, fecal short-chain fatty acids, and the intestinal microbiota in patients with irritable bowel syndrome (IBS) and control volunteers

dc.contributor.authorShin, Andrea
dc.contributor.authorNelson, David
dc.contributor.authorWo, John
dc.contributor.authorCamilleri, Michael
dc.contributor.authorJames-Stevenson, Toyia
dc.contributor.authorSiwiec, Robert
dc.contributor.authorBohm, Matthew
dc.contributor.authorGupta, Anita
dc.contributor.departmentMedicine, School of Medicineen_US
dc.date.accessioned2020-01-13T20:58:30Z
dc.date.available2020-01-13T20:58:30Z
dc.date.issued2018-06
dc.description.abstractOBJECTIVES/SPECIFIC AIMS: Objectives and goals of this study will be to: (1) compare fecal microbiota and fecal organic acids in irritable bowel syndrome (IBS) patients and controls and (2) investigate the association between colonic transit and fecal microbiota in IBS patients and controls. METHODS/STUDY POPULATION: We propose an investigation of fecal organic acids, colonic transit and fecal microbiota in 36 IBS patients and 18 healthy controls. The target population will be adults ages 18–65 years meeting Rome IV criteria for IBS (both diarrhea- and constipation-predominant, IBS-D and IBS-C) and asymptomatic controls. Exclusion criteria are: (a) history of microscopic colitis, inflammatory bowel disease, celiac disease, visceral cancer, chronic infectious disease, immunodeficiency, uncontrolled thyroid disease, liver disease, or elevated AST/ALT>2.0× the upper limit of normal, (b) prior radiation therapy of the abdomen or abdominal surgeries with the exception of appendectomy or cholecystectomy >6 months before study initiation, (c) ingestion of prescription, over the counter, or herbal medications affecting gastrointestinal transit or study interpretation within 6 months of study initiation for controls or within 2 days before study initiation for IBS patients, (d) pregnant females, (e) antibiotic usage within 3 months before study participation, (f) prebiotic or probiotic usage within the 2 weeks before study initiation, (g) tobacco users. Primary outcomes will be fecal bile acid excretion and profile, short-chain fatty acid excretion and profile, colonic transit, and fecal microbiota. Secondary outcomes will be stool characteristics based on responses to validated bowel diaries. Stool samples will be collected from participants during the last 2 days of a 4-day 100 g fat diet and split into 3 samples for fecal microbiota, SCFA, and bile acid analysis and frozen. Frozen aliquots will be shipped to the Metabolite Profiling Facility at Purdue University and the Mayo Clinic Department of Laboratory Medicine and Pathology for SCFA and bile acid measurements, respectively. Analysis of fecal microbiota will be performed in the research laboratory of Dr David Nelson in collaboration with bioinformatics expertise affiliated with the Nelson lab. Colonic transit time will be measured with the previously validated method using radio-opaque markers. Generalized linear models will be used as the analysis framework for comparing study endpoints among groups. RESULTS/ANTICIPATED RESULTS: This study seeks to examine the innovative concept that specific microbial signatures are associated with increased fecal excretion of organic acids to provide unique insights on a potential mechanistic link between altered intraluminal organic acids and fecal microbiota. DISCUSSION/SIGNIFICANCE OF IMPACT: Results may lead to development of targets for novel therapies and diagnostic biomarkers for IBS, emphasizing the role of the fecal metabolome.en_US
dc.eprint.versionFinal published versionen_US
dc.identifier.citationShin, A., Nelson, D., Wo, J., Camilleri, M., James-Stevenson, T., Siwiec, R., … Gupta, A. (2018). 2103 Fecal bile acids, fecal short-chain fatty acids, and the intestinal microbiota in patients with irritable bowel syndrome (IBS) and control volunteers. Journal of Clinical and Translational Science, 2(Suppl 1), 12–13. doi:10.1017/cts.2018.75en_US
dc.identifier.urihttps://hdl.handle.net/1805/21850
dc.language.isoen_USen_US
dc.publisherCambridge University Pressen_US
dc.relation.isversionof10.1017/cts.2018.75en_US
dc.relation.journalJournal of Clinical and Translational Scienceen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.sourcePMCen_US
dc.subjectFecal bile acidsen_US
dc.subjectFecal short-chain fatty acidsen_US
dc.subjectIntestinal microbiotaen_US
dc.subjectIrritable bowel syndromeen_US
dc.subjectAltered intraluminal organic acidsen_US
dc.subjectDiagnostic biomarkersen_US
dc.subjectFecal metabolomeen_US
dc.titleFecal bile acids, fecal short-chain fatty acids, and the intestinal microbiota in patients with irritable bowel syndrome (IBS) and control volunteersen_US
dc.typeArticleen_US
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