Electrospun Azithromycin-Laden Gelatin Methacryloyl Fibers for Endodontic Infection Control

dc.contributor.authorAyoub, Afzan A.
dc.contributor.authorMahmoud, Abdel H.
dc.contributor.authorRibeiro, Juliana S.
dc.contributor.authorDaghrery, Arwa
dc.contributor.authorXu, Jinping
dc.contributor.authorFenno, J. Christopher
dc.contributor.authorSchwendeman, Anna
dc.contributor.authorSasaki, Hajime
dc.contributor.authorDal-Fabbro, Renan
dc.contributor.authorBottino, Marco C.
dc.contributor.departmentBiomedical and Applied Sciences, School of Dentistry
dc.date.accessioned2024-11-25T15:33:50Z
dc.date.available2024-11-25T15:33:50Z
dc.date.issued2022-11-09
dc.description.abstractThis study was aimed at engineering photocrosslinkable azithromycin (AZ)-laden gelatin methacryloyl fibers via electrospinning to serve as a localized and biodegradable drug delivery system for endodontic infection control. AZ at three distinct amounts was mixed with solubilized gelatin methacryloyl and the photoinitiator to obtain the following fibers: GelMA+5%AZ, GelMA+10%AZ, and GelMA+15%AZ. Fiber morphology, diameter, AZ incorporation, mechanical properties, degradation profile, and antimicrobial action against Aggregatibacter actinomycetemcomitans and Actinomyces naeslundii were also studied. In vitro compatibility with human-derived dental pulp stem cells and inflammatory response in vivo using a subcutaneous rat model were also determined. A bead-free fibrous microstructure with interconnected pores was observed for all groups. GelMA and GelMA+10%AZ had the highest fiber diameter means. The tensile strength of the GelMA-based fibers was reduced upon AZ addition. A similar pattern was observed for the degradation profile in vitro. GelMA+15%AZ fibers led to the highest bacterial inhibition. The presence of AZ, regardless of the concentration, did not pose significant toxicity. In vivo findings indicated higher blood vessel formation, mild inflammation, and mature and thick well-oriented collagen fibers interweaving with the engineered fibers. Altogether, AZ-laden photocrosslinkable GelMA fibers had adequate mechanical and degradation properties, with 15%AZ displaying significant antimicrobial activity without compromising biocompatibility.
dc.eprint.versionFinal published version
dc.identifier.citationAyoub AA, Mahmoud AH, Ribeiro JS, et al. Electrospun Azithromycin-Laden Gelatin Methacryloyl Fibers for Endodontic Infection Control. Int J Mol Sci. 2022;23(22):13761. Published 2022 Nov 9. doi:10.3390/ijms232213761
dc.identifier.urihttps://hdl.handle.net/1805/44698
dc.language.isoen_US
dc.publisherMDPI
dc.relation.isversionof10.3390/ijms232213761
dc.relation.journalInternational Journal of Molecular Sciences
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.sourcePMC
dc.subjectAntimicrobial
dc.subjectElectrospinning
dc.subjectDentistry
dc.subjectDrug delivery
dc.subjectEndodontics
dc.subjectFibers
dc.titleElectrospun Azithromycin-Laden Gelatin Methacryloyl Fibers for Endodontic Infection Control
dc.typeArticle
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