In silico identification of microRNAs predicted to regulate the drug metabolizing cytochrome P450 genes

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dc.contributor.authorRamamoorthy, Anuradha
dc.contributor.authorSkaar, Todd C.
dc.contributor.departmentDepartment of Medicine, IU School of Medicineen_US
dc.date.accessioned2016-06-30T12:57:21Z
dc.date.available2016-06-30T12:57:21Z
dc.date.issued2011-04
dc.description.abstractOBJECTIVE: Cytochrome P450 (CYP) enzymes exhibit high interindividual variability that is not completely explained by known environmental and genetic factors. To further understand this variability, we hypothesized that microRNAs (miRNAs) may regulate CYP expression. METHODS: MiRNA identification algorithms were used to identify the miRNAs that are predicted to regulate twelve major drug metabolizing CYPs and to identify polymorphisms in CYP mRNA 3'-UTRs that are predicted to interfere with normal mRNA-miRNA interactions. RESULTS: All twelve CYPs were predicted to be targets of miRNAs. Additionally, 38 SNPs in CYP mRNA 3'-UTRs were predicted to interfere with miRNA targeting of mRNAs. These predicted miRNAs and SNPs are candidates for future in vitro studies focused on understanding the molecular regulation of these CYP genes. CONCLUSION: These in silico results provide strong support for a role of miRNA in the regulation and variability of CYP expression.en_US
dc.eprint.versionAuthor's manuscripten_US
dc.identifier.citationRamamoorthy, A., & Skaar, T. C. (2011). In silico identification of microRNAs predicted to regulate the drug metabolizing cytochrome P450 genes. Drug Metabolism Letters, 5(2), 126–131.en_US
dc.identifier.urihttps://hdl.handle.net/1805/10261
dc.language.isoen_USen_US
dc.publisherBentham Scienceen_US
dc.relation.journalDrug Metabolism Lettersen_US
dc.rightsPublisher Policyen_US
dc.sourcePMCen_US
dc.subjectBioinformatic analysisen_US
dc.subjectCytochrome P450sen_US
dc.subjectMicroRNAen_US
dc.subjectPolymorphismsen_US
dc.titleIn silico identification of microRNAs predicted to regulate the drug metabolizing cytochrome P450 genesen_US
dc.typeArticleen_US
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