A simple automated method for continuous fieldwise measurement of microvascular hemodynamics
dc.contributor.author | Clendenon, Sherry G. | |
dc.contributor.author | Fu, Xiao | |
dc.contributor.author | Von Hoene, Robert A. | |
dc.contributor.author | Clendenon, Jeffrey L. | |
dc.contributor.author | Sluka, James P. | |
dc.contributor.author | Winfree, Seth | |
dc.contributor.author | Mang, Henry | |
dc.contributor.author | Martinez, Michelle | |
dc.contributor.author | Filson, Adele J. | |
dc.contributor.author | Klaunig, James E. | |
dc.contributor.author | Glazier, James A. | |
dc.contributor.author | Dunn, Kenneth W. | |
dc.contributor.department | Medicine, School of Medicine | en_US |
dc.date.accessioned | 2020-07-21T22:03:27Z | |
dc.date.available | 2020-07-21T22:03:27Z | |
dc.date.issued | 2019-05 | |
dc.description.abstract | Microvascular perfusion dynamics are vital to physiological function and are frequently dysregulated in injury and disease. Typically studies measure microvascular flow in a few selected vascular segments over limited time, failing to capture spatial and temporal variability. To quantify microvascular flow in a more complete and unbiased way we developed STAFF (Spatial Temporal Analysis of Fieldwise Flow), a macro for FIJI open-source image analysis software. Using high-speed microvascular flow movies, STAFF generates kymographs for every time interval for every vascular segment, calculates flow velocities from red blood cell shadow angles, and outputs the data as color-coded velocity map movies and spreadsheets. In untreated mice, analyses demonstrated profound variation even between adjacent sinusoids over seconds. In acetaminophen-treated mice we detected flow reduction localized to pericentral regions. STAFF is a powerful new tool capable of providing novel insights by enabling measurement of the complex spatiotemporal dynamics of microvascular flow. | en_US |
dc.eprint.version | Author's manuscript | en_US |
dc.identifier.citation | Clendenon, S. G., Fu, X., Von Hoene, R. A., Clendenon, J. L., Sluka, J. P., Winfree, S., Mang, H., Martinez, M., Filson, A. J., Klaunig, J. E., Glazier, J. A., & Dunn, K. W. (2019). A simple automated method for continuous fieldwise measurement of microvascular hemodynamics. Microvascular research, 123, 7–13. https://doi.org/10.1016/j.mvr.2018.11.010 | en_US |
dc.identifier.uri | https://hdl.handle.net/1805/23309 | |
dc.language.iso | en_US | en_US |
dc.publisher | Elsevier | en_US |
dc.relation.isversionof | 10.1016/j.mvr.2018.11.010 | en_US |
dc.relation.journal | Microvascular Research | en_US |
dc.rights | Publisher Policy | en_US |
dc.source | PMC | en_US |
dc.subject | Red blood cell velocity | en_US |
dc.subject | Intravital microscopy | en_US |
dc.subject | Hemodynamics | en_US |
dc.subject | Microvascular | en_US |
dc.subject | Capillary | en_US |
dc.title | A simple automated method for continuous fieldwise measurement of microvascular hemodynamics | en_US |
dc.type | Article | en_US |