Browsing by Subject "volumetric muscle loss"

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    Myogenic tissue nanotransfection improves muscle torque recovery following volumetric muscle loss
    (Nature, 2022) Clark, Andrew; Ghatak, Subhadip; Guda, Poornachander Reddy; El Masry, Mohamed S.; Xuan, Yi; Sato, Amy Y.; Bellido, Teresita; Sen, Chandan K.; Surgery, School of Medicine
    This work rests on our non-viral tissue nanotransfection (TNT) platform to deliver MyoD (TNT) to injured tissue in vivo. TNT was performed on skin and successfully induced expression of myogenic factors. TNT was then used as a therapy 7 days following volumetric muscle loss (VML) of rat tibialis anterior and rescued muscle function. TNT is promising as VML intervention.
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    Severe muscle trauma triggers heightened and prolonged local musculoskeletal inflammation and impairs adjacent tibia fracture healing
    (2016-06) Hurtgen, B. J.; Ward, C. L.; Garg, K.; Pollot, B. E.; Goldman, S. M.; Mckinley, Todd O.; Wenke, J. C.; Corona, B. T.; Department of Orthopaedic Surgery, IU School of Medicine
    Objectives: Complicated fracture healing is often associated with the severity of surrounding muscle tissue trauma. Since inflammation is a primary determinant of musculoskeletal health and regeneration, it is plausible that delayed healing and non-unions are partly caused by compounding local inflammation in response to concomitant muscle trauma. Methods and results: To investigate this possibility, a Lewis rat open fracture model [tibia osteotomy with adjacent tibialis anterior (TA) muscle volumetric muscle loss (VML) injury] was interrogated. We observed that VML injury impaired tibia healing, as indicated by diminished mechanical strength and decreased mineralized bone within the fracture callus, as well as continued presence of cartilage instead of woven bone 28 days post-injury. The VML injured muscle presented innate and adaptive immune responses that were atypical of canonical muscle injury healing. Additionally, the VML injury resulted in a perturbation of the inflammatory phase of fracture healing, as indicated by elevations of CD3+ lymphocytes and CD68+ macrophages in the fracture callus at 3 and 14d post-injury, respectively. Conclusions: These data indicate that heightened and sustained innate and adaptive immune responses to traumatized muscle are associated with impaired fracture healing and may be targeted for the prevention of delayed and non-union following musculoskeletal trauma.