Browsing by Subject "Matrigel"

Now showing 1 - 1 of 1
  • Thumbnail Image
    Item
    Production of Single Contracting Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes: Matrigel Mattress Technique
    (Wiley, 2017-08-14) Cadar, Adrian G.; Feaster, Tromondae K.; Durbin, Matthew D.; Hong, Charles C.; Pediatrics, School of Medicine
    This unit describes the published Matrigel mattress method. Briefly, we describe the preparation of the mattress, replating of the human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) on the Matrigel mattress, and hiPSC-CM mattress maintenance. Adherence to this protocol will yield individual, robustly shortening hiPSC-CMs, which can be used for downstream applications.