Browsing by Subject "3D models"

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    3D Bone Morphology Alters Gene Expression, Motility, and Drug Responses in Bone Metastatic Tumor Cells
    (MDPI, 2020-09-21) Dadwal, Ushashi C.; Merkel, Alyssa R.; Page, Jonathan M.; Kwakwa, Kristin A.; Kessler, Michael; Rhoades, Julie A.; Anatomy and Cell Biology, School of Medicine
    Patients with advanced skeletal metastases arising from primary cancers including breast, lung, and prostate suffer from extreme pain, bone loss, and frequent fractures. While the importance of interactions between bone and tumors is well-established, our understanding of complex cell–cell and cell–microenvironment interactions remains limited in part due to a lack of appropriate 3D bone models. To improve our understanding of the influence of bone morphometric properties on the regulation of tumor-induced bone disease (TIBD), we utilized bone-like 3D scaffolds in vitro and in vivo. Scaffolds were seeded with tumor cells, and changes in cell motility, proliferation, and gene expression were measured. Genes associated with TIBD significantly increased with increasing scaffold rigidity. Drug response differed when tumors were cultured in 3D compared to 2D. Inhibitors for Integrin β3 and TGF-β Receptor II significantly reduced bone-metastatic gene expression in 2D but not 3D, while treatment with the Gli antagonist GANT58 significantly reduced gene expression in both 2D and 3D. When tumor-seeded 3D scaffolds were implanted into mice, infiltration of myeloid progenitors changed in response to pore size and rigidity. This study demonstrates a versatile 3D model of bone used to study the influence of mechanical and morphometric properties of bone on TIBD.
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    Accuracy of 3D Reconstructed Orthodontic Models
    (2014) Stewart, Kelton; Chai, Billy; Liu, Sean; Ghoneima, Ahmed; Alford, Timothy
    Objective: To evaluate the accuracy of 3D reconstructed orthodontic models, derived by various digitization methods, as compared to conventional orthodontic plaster models. Materials and Methods: Twenty-five maxillary orthodontic plaster models were randomly selected from the Indiana University School of Dentistry Department of Orthodontics. Each plaster model was scanned with the Cadent iOC scanner and the digital data was used to print 3D reconstructed orthodontic models. The same 25 plaster models were duplicated using alginate and poured in plaster after two days. These duplicated plaster models were also scanned with the iOC scanner and 3D reconstructed. Next, the duplicate plaster models were sent to a lab, scanned with a 3Shape R700 scanner, and the digital data was 3D reconstructed. Digital calipers were used to obtain ten linear dimensional measurements on all plaster and 3D reconstructed models for comparisons. Equivalence testing was performed using 2 one-sided paired t-tests with a significance level of P <0.05. Results: Nine of the 10 linear measurements were statistically equivalent in all groups. Clinically insignificant, but statistically significant, measurement differences in maxillary central incisor height (P <0.05) were found on 3D reconstructed models derived from the 3Shape R700 desktop scanner. Conclusion: 3D reconstructed orthodontic models derived from alginate impressions, iOC scanners, and 3Shape R700 scanners are an accurate and reliable substitute for orthodontic plaster models.