(Office of the Vice Chancellor for Research, 2013-04-05) AlZain, Afnan; Eckert, George; Platt, Jeffrey A.
This study evaluated the microtensile dentin bond strength (μ-TBS) and microleakage of a one-step
HEMA-free self-etch adhesive (G-aenial Bond-GB) compared to a 2-step self-etch (Clearfil SE-SE) and a
3-step etch and rinse (OptiBond FL-OB) adhesive with and without pre-etching with phosphoric acid.
Human molars were divided randomly into 5 groups (n=15), GB and SE (without pre-etching), GB+ and
SE+ (with pre-etching), and OB. Eight beams were obtained from each tooth and half of the beams were
subjected to μ-TBS testing after 2d. The remaining beams were thermocycled (2,500 cycles), aged for
40d and subjected to μ-TBS testing using a universal testing machine. Failures were analyzed using light
microscopy and SEM. Similar groups were used for the microleakage test (n=11). Class V cavities were
prepared on the buccal and lingual surfaces of each molar. Teeth were thermocycled (2,500 cycles), aged
for 40d, soaked in 1% methylene blue dye for 24h, and sectioned longitudinally from the facial to lingual
surface. The dye penetration was scored using light microscopy and an ordinal scale from 0-3. Data was
analyzed using Weibull, GEE, and Wilcoxon Rank Sum tests (α=0.05). Phosphoric acid pre-etching
significantly increased dentin bond strength. After 40d, the mean bond strength ranged from 28.6-45.7
MPa with a statistical significance of GB, SE