Browsing by Author "Rand, R. Peter"

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    Elastic Properties of Polyunsaturated Phosphatidylethanolamines Influence Rhodopsin Function
    (Royal Society of Chemistry, 2013) Teague, Walter E., Jr.; Soubias, Olivier; Petrache, Horia; Fuller, Nola; Hines, Kirk G.; Rand, R. Peter; Gawrisch, Klaus; Physics, School of Science
    Membranes with a high content of polyunsaturated phosphatidylethanolamines (PE) facilitate formation of metarhodopsin-II (M(II)), the photointermediate of bovine rhodopsin that activates the G protein transducin. We determined whether M(II)-formation is quantitatively linked to the elastic properties of PEs. Curvature elasticity of monolayers of the polyunsaturated lipids 18 : 0-22 : 6(n - 3)PE, 18 : 0-22 : 5(n)- 6PE and the model lipid 18 : 1(n - 9)-18 : 1,(n- 9)PE were investigated in the inverse hexagonal phase. All three lipids form lipid monolayers with rather low spontaneous radii of curvature of 26-28 angstroms. In membranes, all three PEs generate high negative curvature elastic stress that shifts the equilibrium of MI(I)/M(II) photointermediates of rhodopsin towards M(II) formation.