Browsing by Author "Nicola, Anthony V."

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    Molecular Requirement for Sterols in Herpes Simplex Virus Entry and Infectivity
    (American Society for Microbiology (ASM), 2014-12) Wudiri, George A.; Pritchard, Suzanne M.; Li, Hong; Liu, Jin; Aguilar, Hector C.; Gilk, Stacey D.; Nicola, Anthony V.; Department of Microbiology & Immunology, IU School of Medicine
    Herpes simplex virus 1 (HSV-1) required cholesterol or desmosterol for virion-induced membrane fusion. HSV successfully entered DHCR24−/− cells, which lack a desmosterol-to-cholesterol conversion enzyme, indicating that entry can occur independently of cholesterol. Depletion of desmosterol from these cells resulted in diminished HSV-1 entry, suggesting a general sterol requirement for HSV-1 entry and that desmosterol can operate in virus entry. Cholesterol functioned more effectively than desmosterol, suggesting that the hydrocarbon tail of cholesterol influences viral entry.