Browsing by Author "McCloskey, Duncan"

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    LAMP-enabled diagnosis of Kaposi's sarcoma for sub-Saharan Africa
    (American Association for the Advancement of Science, 2023) McCloskey, Duncan; Semeere, Aggrey; Ayanga, Racheal; Laker-Oketta, Miriam; Lukande, Robert; Semakadde, Matthew; Kanyesigye, Micheal; Wenger, Megan; LeBoit, Philip; McCalmont, Timothy; Maurer, Toby; Gardner, Andrea; Boza, Juan; Cesarman, Ethel; Martin, Jeffrey; Erickson, David; Dermatology, School of Medicine
    Kaposi's sarcoma (KS) is an endothelial cancer caused by the Kaposi's sarcoma-associated herpesvirus (KSHV) and is one of the most common cancers in sub-Saharan Africa. In limited-resource settings, traditional pathology infrastructure is often insufficient for timely diagnosis, leading to frequent diagnoses at advanced-stage disease where survival is poor. In this study, we investigate molecular diagnosis of KS performed in a point-of-care device to circumvent the limited infrastructure for traditional diagnosis. Using 506 mucocutaneous biopsies collected from patients at three HIV clinics in Uganda, we achieved 97% sensitivity, 92% specificity, and 96% accuracy compared to gold standard U.S.-based pathology. The results presented in this manuscript show that LAMP-based quantification of KSHV DNA extracted from KS-suspected biopsies has the potential to serve as a successful diagnostic for the disease and that diagnosis may be accurately achieved using a point-of-care device, reducing the barriers to obtaining KS diagnosis while increasing diagnostic accuracy.