Browsing by Author "Harbin, Sherry L Voytik"

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    Monitoring focal adhesion kinase phosphorylation dynamics in live cells
    (Royal Society of Chemistry, 2017-07-24) Damayanti, Nur P.; Buno, Kevin; Narayanan, Nagarajan; Harbin, Sherry L Voytik; Deng, Meng; Irudayaraj, Joseph M.K.; Medicine, School of Medicine
    Focal adhesion kinase (FAK) is a cytoplasmic non-receptor tyrosine kinase essential for a diverse set of cellular functions. Current methods for monitoring FAK activity in response to an extracellular stimulus lack spatiotemporal resolution and/or the ability to perform multiplex detection. Here we report on a novel approach to monitor the real-time kinase phosphorylation activity of FAK in live single cells by fluorescence lifetime imaging.