Browsing by Author "DeBosch, Brian J."

Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    A New Tool to Study the DREADDed Peripheral Nervous System
    (Elsevier, 2025) Alhadeff, Amber L.; DeBosch, Brian J.; Pediatrics, School of Medicine
  • Thumbnail Image
    Item
    Protocol to evaluate fasting metabolism and its relationship to the core circadian clock in mice
    (Elsevier, 2025) Sun, Jiameng; DeBosch, Brian J.; Pediatrics, School of Medicine
    To investigate the role of hepatocyte genes in fasting metabolism, it is essential to analyze their expression across the entire 24-h circadian cycle. Here, we present a protocol to evaluate fasting metabolism and its relationship to the core circadian clock in mice. We describe steps for time-course fasting/feeding experiment setup and performing staggered fasting and refeeding for 24 h. We then detail procedures for evaluating oxidative substrate selection and rescuing defective oxidative metabolism through FGF21 and CPI-613 injections. For complete details on the use and execution of this protocol, please refer to Sun et al.