Browsing by Author "Alakhras, Nada S."

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    A method to isolate the CTD of RNA Polymerase II for proteomics analysis
    (2014-12) Alakhras, Nada S.; Mosley, Amber L.; Goebl, Mark G.; Hurley, Thomas D., 1961-
    In an effort to advance the methodology in analyzing RNAPII protein-protein interaction network and to determine the role of the CTD in controlling RNAPII transcription, we devised a method to specifically isolate the CTD-associated and CTD-less RNAPII to identify the proteins that interact with both the CTD and the globular core of RNAPII using novel purification scheme coupled to quantitative proteomics.