Nutrition and Dietetics Works

Permanent URI for this collection

https://hdl.handle.net/1805/5908

Browse

Browsing Nutrition and Dietetics Works by Author "Chakraborty, Sujata"

Now showing 1 - 1 of 1
  • Thumbnail Image
    Item
    Cytogenetic features of human trophoblast cell lines SWAN-71 and 3A-subE
    (Elsevier, 2017-04) Reiter, Jill L.; Drendel, Holli M.; Chakraborty, Sujata; Schellinger, Megan M.; Lee, Men-Jean; Mor, Gil; Department of Obstetrics and Gynecology, IU School of Medicine
    Immortalization of primary cells with telomerase is thought to maintain normal phenotypic properties and avoid chromosomal abnormalities and other cancer-associated changes that occur following simian virus 40 tumor antigen (SV40 Tag) induced immortalization. However, we report that the human telomerase reverse transcriptase (hTERT)-immortalized SWAN-71 trophoblast cell line has a near pentaploid 103∼119,XXXX[cp20] karyotype. Additionally, DNA typing analysis indicated that SWAN-71 cells have acquired microsatellite instability. In comparison, the post-crisis SV40-transformed trophoblast cell line 3A-subE was hypertriploid 69∼81,XX[cp20]. Both cell lines contained multiple specific clonal rearrangements. These findings emphasize the need to monitor for genetic instability in hTERT-immortalized cells.