The role of the Borrelia oxidative stress regulator protein in virulence gene expression of the Lyme disease spirochete

dc.contributor.advisorYang, X. Frank
dc.contributor.authorKhoo, Joleyn Yean Chern
dc.contributor.otherSpinola, Stanley M.
dc.contributor.otherBauer, Margaret E.
dc.contributor.otherJohnson, Raymond M. of Microbiology and Immunologyen Universityen_US
dc.descriptionIndiana University-Purdue University Indianapolis (IUPUI)en_US
dc.description.abstractThe Lyme disease agent, Borrelia burgdorferi, has a complex system that allows it to thrive in the harsh and distinct environments of its tick vector and mammalian host. Although it has been known for some time that the Borrelia oxidative stress regulator protein (BosR) plays a necessary role in mammalian infectivity and functions as a transcriptional regulator of alternative sigma factor RpoS, very little is known about its mechanism of action, other than the suggestion that BosR activates rpoS transcription by binding to certain upstream regions of the gene. In our studies, we performed protein degradation assays and luciferase reporter assays for further understanding of BosR function. Our preliminary findings suggest that BosR is post-transcriptionally regulated by an unknown protease and may not need to bind to any rpoS upstream regions in order to activate transcription. We also describe the construction of luciferase reporter systems that will shed light on BosR’s mechanism of action. We postulate the provocative possibility that unlike its homologs Fur and PerR in other bacterial systems, BosR may not utilize a DNA-binding mechanism in order to fulfill its role as a transcriptional regulator to modulate virulence gene expression.en_US
dc.subjectLyme disease, Borrelia burgdorferi, BosR, RpoSen_US
dc.subject.lcshLyme disease -- Research -- Analysisen_US
dc.subject.lcshBorrelia burgdorferi -- Researchen_US
dc.subject.lcshGenetic regulationen_US
dc.subject.lcshGene expression -- Techniqueen_US
dc.subject.lcshGenetic transcription -- Regulationen_US
dc.subject.lcshProtease inhibitorsen_US
dc.subject.lcshVirulence (Microbiology)en_US
dc.subject.lcshRNA polymerasesen_US
dc.subject.lcshPolymerase chain reactionen_US
dc.subject.lcshTicks as carriers of diseaseen_US
dc.subject.lcshMessenger RNA -- Research -- Analysis -- Evaluationen_US
dc.subject.lcshMicrobial genetics -- Techniqueen_US
dc.subject.lcshPolyacrylamide gel electrophoresisen_US
dc.titleThe role of the Borrelia oxidative stress regulator protein in virulence gene expression of the Lyme disease spirocheteen_US
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