Integrative Click Chemistry for Tuning Physicochemical Properties of Cancer Cell-Laden Hydrogels

dc.contributor.advisorLin, Chien-Chi
dc.contributor.authorJohnson, Hunter C.
dc.contributor.otherNaumann, Christoph
dc.contributor.otherNa, Sungsoo
dc.date.accessioned2020-05-01T18:11:58Z
dc.date.available2020-05-01T18:11:58Z
dc.date.issued2020-05
dc.degree.date2020en_US
dc.degree.disciplineBiomedical Engineering
dc.degree.grantorPurdue Universityen_US
dc.degree.levelM.S.en_US
dc.descriptionIndiana University-Purdue University Indianapolis (IUPUI)en_US
dc.description.abstractThe pancreas is a vital organ that secretes key metabolic hormones and digestive enzymes. In pancreatic ductal adenocarcinoma (PDAC), one of the leading causes of cancer-related death in the world, limited advances in diagnosis or therapies have been made over decades. Key features of PDAC progression include an elevated matrix sti ness and an increased deposition of extracellular matrices (ECM), such as hyaluronic acid (HA). Understanding how cells interact with components in the tumor microenvironment (TME) as PDAC progresses can assist in developing diagnostic tools and therapeutic treatment options. In recent years, hydrogels have proven to be an excellent platform for studying cell-cell and cell-matrix interactions. Utilizing chemically modi ed and naturally derived materials, hydrogel networks can be formed to encompass not only the components, but also the physicochemical properties of the dynamic TME. In this work, a dynamic hydrogel system that integrates multiple click chemistries was developed for tuning matrix physicochemical properties in a manner similar to the temporally increased matrix sti ness and depositions of HA. Subsequently, these dynamic hydrogels were used to investigate how matrix sti ening and increased HA presentation might a ect survival of PDAC cells and their response to chemotherapeutics.en_US
dc.identifier.urihttps://hdl.handle.net/1805/22677
dc.identifier.urihttp://dx.doi.org/10.7912/C2/1373
dc.language.isoenen_US
dc.subjectHydrogelen_US
dc.subjectCanceren_US
dc.subjectPDACen_US
dc.subjectBiomimeticen_US
dc.subjectStiffnessen_US
dc.subjectHyaluronic Aciden_US
dc.titleIntegrative Click Chemistry for Tuning Physicochemical Properties of Cancer Cell-Laden Hydrogelsen_US
dc.typeThesis
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