Physisorbed Polymer-Tethered Lipid Bilayer with Lipopolymer Gradient

dc.contributor.authorLin, Yu-Hung
dc.contributor.authorMinner, Daniel E.
dc.contributor.authorHerring, Vincent L.
dc.contributor.authorNaumann, Christoph A.
dc.contributor.departmentChemistry and Chemical Biology, School of Scienceen_US
dc.date.accessioned2018-05-15T20:06:19Z
dc.date.available2018-05-15T20:06:19Z
dc.date.issued2012-11
dc.description.abstractPhysisorbed polymer-tethered lipid bilayers consisting of phospholipids and lipopolymers represent an attractive planar model membrane platform, in which bilayer fluidity and membrane elastic properties can be regulated through lipopolymer molar concentration. Herein we report a method for the fabrication of such a planar model membrane system with a lateral gradient of lipopolymer density. In addition, a procedure is described, which leads to a sharp boundary between regions of low and high lipopolymer molar concentrations. Resulting gradients and sharp boundaries are visualized on the basis of membrane buckling structures at elevated lipopolymer concentrations using epifluorescence microscopy and atomic force microscopy. Furthermore, results from spot photobleaching experiments are presented, which provide insight into the lipid lateral fluidity in these model membrane architectures. The presented experimental data highlight a planar, solid-supported membrane characterized by fascinating length scale-dependent dynamics and elastic properties with remarkable parallels to those observed in cellular membranes.en_US
dc.eprint.versionFinal published versionen_US
dc.identifier.citationLin, Y.-H., Minner, D. E., Herring, V. L., & Naumann, C. A. (2012). Physisorbed Polymer-Tethered Lipid Bilayer with Lipopolymer Gradient. Materials, 5(11), 2243–2257. http://doi.org/10.3390/ma5112243en_US
dc.identifier.urihttps://hdl.handle.net/1805/16190
dc.language.isoen_USen_US
dc.publisherMDPIen_US
dc.relation.isversionof10.3390/ma5112243en_US
dc.relation.journalMaterialsen_US
dc.rightsAttribution 3.0 United States
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/us
dc.sourcePMCen_US
dc.subjectGradient bilayeren_US
dc.subjectLangmuir-Blodgett transferen_US
dc.subjectLipopolymeren_US
dc.subjectPolymer-tethered lipid membraneen_US
dc.subjectEpifluorescenceen_US
dc.subjectAtomic force microscopyen_US
dc.subjectBuckling structureen_US
dc.titlePhysisorbed Polymer-Tethered Lipid Bilayer with Lipopolymer Gradienten_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
materials-05-02243.pdf
Size:
5.82 MB
Format:
Adobe Portable Document Format
Description:
Main article
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.99 KB
Format:
Item-specific license agreed upon to submission
Description: