Anti-angiogenic activity of kidney derived endothelial cells

If you need an accessible version of this item, please submit a remediation request.
Date
2013-04-05
Language
American English
Embargo Lift Date
Department
Committee Members
Degree
Degree Year
Department
Grantor
Journal Title
Journal ISSN
Volume Title
Found At
Office of the Vice Chancellor for Research
Abstract

The identification of novel endogenous mediators of angiogenic/vasculogenic processes may provide for novel therapeutic targets to modulate blood vessel growth in disease states, such as cardiovascular disease or cancer. Studies in our lab have shown that blood vessels in kidney have little endogenous regenerative capacity. Kidney derived microvascular endothelial cells (KEC) were isolated from rat kidney or from transgenic mice bearing the temperature sensitive SV40 mutant (and subsequently grown at non-permissive temperature, 37oC). Both rat and mouse KECs manifested significantly reduced growth rates when compared with several commonly used EC lines (rat pulmonary EC, HUVEC and human cord blood colony forming ECs). In 2D matrigel assays, all commonly used ECs faithfully formed characteristic branching structures; while all KECs failed to form stabile branching structures. Time-course analysis of branching activity demonstrated that KEC initially formed primitive branching nodes within 3 hours of culture, but these structures regressed such that no branched structures were observed between 6-12 hours. Co-culture of KECs with any branching competent EC impaired branching dose dependently. When co-cultured with ECFC, labeled KECs incorporated into primitive ECFC branches within the first 3 hours of plating. However, when compared with ECFC branches, ECFC-KEC mixed branches showed a more rapid regression of the branched structures between 12-24 hrs. Interestingly, conditioned media from KEC did not affect branching of competent ECFC. Taken together, these data indicate that KEC have anti-angiogenic activity that may destabilize ECs during angiogenesis. The anti-angiogenic activity requires cell-cell contact, suggesting the possible presence of an angio-inhibitory molecule on the cell surface of KECs. Current and future studies seek to generate additional KEC lines, and will determine if KEC cell fractions mediate the anti-angiogenic effect. In addition, we will seek to determine if KECs mitigate progression of angiogenic dependent tumor formation in vivo.

Description
poster abstract
item.page.description.tableofcontents
item.page.relation.haspart
Cite As
Basile, David P., Coleen Mallet, and Mervin C. Yoder. (2013, April 5). Anti-angiogenic activity of kidney derived endothelial cells. Poster session presented at IUPUI Research Day 2013, Indianapolis, Indiana.
ISSN
Publisher
Series/Report
Sponsorship
Major
Extent
Identifier
Relation
Journal
Source
Alternative Title
Type
Presentation
Number
Volume
Conference Dates
Conference Host
Conference Location
Conference Name
Conference Panel
Conference Secretariat Location
Version
Full Text Available at
This item is under embargo {{howLong}}