Interactive Effects of Elevated CO2 and Salinity on Three Common Grass Species

Abstract

Carbon dioxide (CO2) level in the atmosphere has increased steadily since Pre-Industrial times. The need for a better understanding of the effects of elevated CO2 on plant physiology and growth is clear. Previous studies have focused on how plants are affected by either elevated CO2 or salinity, one of many environmental stresses for plants. However, little research has been focused on the interaction of these two factors. In my project, three common grass species were exposed to both elevated CO2 and salinity, so that the effects of either of these factors and the interaction of the two on these species could be examined. The CO2 levels were set to 400 µmol mol-1, close to the current concentration, or 760 µmol mol-1, projected to be reached by the end of this century. Salt solutions of 0, 25, 50, 75, and 100 mM NaCl with CaCl2 at lower rates (1% of each respective molarity for NaCl) were used to water the grasses, which are unlikely to experience prolonged exposure to salt conditions beyond this range in their natural habitats. The three common grass species studied in my experiment were Kentucky bluegrass (Poa pratensis L.) and red fescue (Festuca rubra L.), both C3 cool season grasses, as well as buffalo grass (Buchloe dactyloides (Nutt.) Engelm.), a C4 warm season grass. Each treatment had five replicates, bringing the total number of experimental pots to 150. Various growth parameters were monitored, and all data was statistically analyzed for statistical significance. My results showed that elevated CO2 had a stimulating effect on most growth parameters, particularly when plants were given more time to grow. In a 100-day growth experiment, CO2 affected the number and dry biomass of plants of all species, regardless of their C3 or C¬4 photosynthetic pathways. Salinity consistently inhibited germination and growth at all stages, from germination through plant emergences, numbers of established plants, and dry biomasses at harvest. Interactive effects of CO2 and salinity did occur, though often in seemingly specific instances rather than forming clear and consistent trends. My findings suggested that growth of common grasses would be enhanced by the rising level of CO2 in the atmosphere, but the effect would be modified by environmental stresses, such as salinity.