INTERACTIONS OF HUMAN GINGIVAL FIBROBLASTS WITH TOBACCO TREATED PORPHYROMONAS GINGIVALIS

dc.contributor.authorLanier, Branden
dc.contributor.authorAl-Shibani, Nouf Khider
dc.contributor.authorWindsor, L. Jack
dc.contributor.authorGregory, Richard
dc.date.accessioned2015-12-16T19:47:05Z
dc.date.available2015-12-16T19:47:05Z
dc.date.issued2012-04-13
dc.descriptionposter abstracten_US
dc.description.abstractPorphyromonas gingivalis (P. gingivalis) and tobacco are risk factors for periodontal disease. The objective of this study was to determine the effects that tobacco treated P. gingivalis cells have on human gingival fibroblasts (HGFs). The study was conducted to examine the effects that cigarette smoke condensate (CSC), nicotine, and dissolvable smokeless tobacco (DST) strips treated P. gingivalis has on cell cytotoxicity and the expression of cy-tokines and growth factors from HGFs. The P. gingivalis was grown at 37°C and then the cells and supernatant were separated. P. gingivalis cells were then washed and killed. The concentration of protein in the cell pellet and supernatant were determined by protein assay using the Bradford method. The lowest non-toxic levels of the cell pellet and supernatant will be used to treat the HGFs for 72 hours and then cytotoxicity was determined by lactate dehydrogenase (LDH) assays. The cytokine/growth factor expression will be determined by antibody protein arrays. The protein assays showed that the tobacco products reduced the protein amounts as compared to untreated bacteria. The results should show an increase in cytotoxicity with increasing protein concentrations, along with increased pro-inflammatory cyto-kine/growth factors expression by the HGFs treated with tobacco treated P. gingivalis compared to P. gingivalis that was not treated with tobacco prod-ucts. A better understanding of the detrimental effects that tobacco has on the underlining causes of periodontal disease can advance the quest of con-trolling the disease. This study was funded by the Indiana University–Purdue University Indianapolis Multidisci-plinary Undergraduate Research Institute (MURI).en_US
dc.identifier.citationBranden Lanier, Nouf Khider Al-Shibani, L. Jack Windsor and Richard Gregory.(2012, April 13). INTERACTIONS OF HUMAN GINGIVAL FIBROBLASTS WITH TOBACCO TREATED PORPHYROMONAS GINGIVALIS. Poster session presented at IUPUI Research Day, 2012, Indianapolis, Indiana.en_US
dc.identifier.urihttps://hdl.handle.net/1805/7759
dc.language.isoen_USen_US
dc.publisherOffice of the Vice Chancellor for Researchen_US
dc.subjecttobaccoen_US
dc.subjectperiodontal diseaseen_US
dc.subjectPorphyromonas gingivalis (P. gingivalis)en_US
dc.subjecthuman gingival fibroblasts (HGFs)en_US
dc.titleINTERACTIONS OF HUMAN GINGIVAL FIBROBLASTS WITH TOBACCO TREATED PORPHYROMONAS GINGIVALISen_US
dc.typePosteren_US
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Lanier-Interactions.pdf
Size:
13.08 KB
Format:
Adobe Portable Document Format
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.88 KB
Format:
Item-specific license agreed upon to submission
Description: