MultiBac: expanding the research toolbox for multiprotein complexes

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Date
2012-02
Authors
Bieniossek, Christoph
Imasaki, Tsuyoshi
Takagi, Yuichiro
Berger, Imre
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English
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Biochemistry and Molecular Biology, School of Medicine
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Elsevier
Abstract

Protein complexes composed of many subunits carry out most essential processes in cells and, therefore, have become the focus of intense research. However, deciphering the structure and function of these multiprotein assemblies imposes the challenging task of producing them in sufficient quality and quantity. To overcome this bottleneck, powerful recombinant expression technologies are being developed. In this review, we describe the use of one of these technologies, MultiBac, a baculovirus expression vector system that is particularly tailored for the production of eukaryotic multiprotein complexes. Among other applications, MultiBac has been used to produce many important proteins and their complexes for their structural characterization, revealing fundamental cellular mechanisms.

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This article is made available for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
Keywords
MultiBac, multiprotein complexes, cellular function
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Bieniossek, C., Imasaki, T., Takagi, Y., & Berger, I. (2012). MultiBac: expanding the research toolbox for multiprotein complexes. Trends in biochemical sciences, 37(2), 49-57. https://doi.org/10.1016/j.tibs.2011.10.005
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Trends in biochemical sciences
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Publisher Policy,This article is made available for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
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https://hdl.handle.net/1805/22702
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https://doi.org/10.1016/j.tibs.2011.10.005
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