Transcriptional Regulation of Retinal Progenitor Cells Derived from Human Induced Pluripotent Stem Cells.

dc.contributor.advisorMeyer, Jason S.
dc.contributor.authorSridhar, Akshayalakshmi
dc.contributor.otherMarrs, James
dc.contributor.otherBelecky-Adams, Teri
dc.date.accessioned2013-08-22T18:59:35Z
dc.date.available2013-08-22T18:59:35Z
dc.date.issued2013-08-22
dc.degree.date2012en_US
dc.degree.disciplineDepartment of Biologyen_US
dc.degree.grantorPurdue Universityen_US
dc.degree.levelM.S.en_US
dc.descriptionIndiana University-Purdue University Indianapolis (IUPUI)en_US
dc.description.abstractIn order to develop effective cures for diseases and decipher disease pathology, the need exists to cultivate a better understanding of human development. Existing studies employ the use of animal models to study and model human development and disease phenotypes but the evolutionary differences between humans and other species slightly limit the applicability of such animal models to effectively recapitulate human development. With the development of human pluripotent stem cells (hPSCs), including Human induced Pluripotent stem cells (hiPSCs) and Human Embryonic Stem cells (hESCs), human development can now be mirrored and recapitulated in vitro. These stem cells are pluripotent, that is, they possess the potential to generate any cell type of the body including muscle cells, nerve cells or blood cells. One of the major focuses of this study is to use hiPSCs to better understand and model human retinogenesis. The retina develops within the first three months of human development, hence rendering it inaccessible to investigation via traditional methods. However, with the advent of hiPSCs, retinal cells can be generated in a culture dish and the mechanisms underlying the specification of a retinal fate can be determined. Additionally, in order to use hiPSCs for successful cell replacement therapy, non-xenogeneic conditions need to be employed to allow for fruitful transplantation tests. Hence, another emphasis of this study has been to direct hiPSCs to generate retinal cells under non-xenogeneic conditions to facilitate their use for future translation purposes.en_US
dc.identifier.urihttps://hdl.handle.net/1805/3454
dc.identifier.urihttp://dx.doi.org/10.7912/C2/2150
dc.language.isoen_USen_US
dc.subjectStem Cell, Human, iPS, Differentiation, Development, Retinaen_US
dc.subject.lcshStem cellsen_US
dc.subject.lcshMultipotent stem cellsen_US
dc.subject.lcshGenetic engineeringen_US
dc.subject.lcshEmbryonic stem cellsen_US
dc.subject.lcshRegenerative medicine -- Researchen_US
dc.subject.lcshCell differentiation -- Molecular aspectsen_US
dc.subject.lcshRetina -- Molecular aspectsen_US
dc.subject.lcshRetina -- Differentiationen_US
dc.subject.lcshDevelopmental biologyen_US
dc.titleTranscriptional Regulation of Retinal Progenitor Cells Derived from Human Induced Pluripotent Stem Cells.en_US
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
AS thesis with changes.pdf
Size:
116.13 MB
Format:
Adobe Portable Document Format
Description:
full thesis with abstract
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.88 KB
Format:
Item-specific license agreed upon to submission
Description: