Effects of DynaMatrix® on angiogenic cytokine expression from human dental pulp fibroblasts : an in vitro study

dc.contributor.advisorSpolnik, Kenneth Jacob, 1950-
dc.contributor.authorAdams, Joseph Benjamin
dc.contributor.otherErhlich, Ygal
dc.contributor.otherBringas, Josef
dc.contributor.otherWarner, Ned A. (Ned Alan)
dc.contributor.otherZunt, Susan L., 1951-
dc.contributor.otherWindsor, L. Jack
dc.date.accessioned2015-06-24T00:14:42Z
dc.date.available2015-06-24T00:14:42Z
dc.date.issued2015
dc.degree.date2015en_US
dc.degree.disciplineSchool of Dentistryen
dc.degree.grantorIndiana Universityen_US
dc.degree.levelM.S.D.en_US
dc.descriptionIndiana University-Purdue University Indianapolis (IUPUI)en_US
dc.description.abstractEFFECTS OF DYNAMATRIX® ON ANGIOGENIC CYTOKINE EXPRESSION FROM HUMAN DENTAL PULP FIBROBLASTS: AN IN VITRO STUDY by Joseph Benjamin Adams Indiana University School of Dentistry Indianapolis, IN Introduction: An exogenous scaffold may lead to more predictable pulp tissue regeneration and continued root formation in a regenerative endodontic procedure. DynaMatrix® is a natural membrane scaffold made of porcine small intestine, currently used in periodontal regenerative surgeries. Objective: The purpose of this study was to investigate if human dental pulp fibroblasts (HDPFs) seeded on DynaMatrix® membrane would result in an increase in the expression of angiogenic cytokines. Materials and Methods: HDPFs (75,000 per well) were seeded in 6-well plates. Three groups were tested: Group 1 (C): HDPFs in 70 media only; Group 2 (M): DynaMatrix® (Cook Biotech, Indianapolis, IN) alone in media; and Group 3 (C+M): HDPFs seeded on DynaMatrix® membranes. After 72 hours of incubation in serum positive, the conditioned media were collected and analyzed for the expression of 20 angiogenic cytokines utilizing RayBiotech Inc., arrays per the manufacturer’s instruction. The data were analyzed by ANOVA. Results: Group M was significantly higher than C for bFGF (p = 0.0023). C+M was significantly higher than M for ANG (p = 0.0104); GRO (p = 0.0003); IFN-γ (p = 0.0023); IL-6 (p = 0.0003); IL-8 (p = 0.0003); Leptin (p = 0.0003); MCP-1 (p = 0.0104); TIMP-1 (p = 0.0190); TIMP-2 (0.0123). C was significantly higher than C+M for ANG (p = 0.0104); MCP-1 (p = 0.0104); and THPO (p = 0.0308). Cytokines such as b-FGF, ANG, and leptin promote angiogenesis, and stimulate migration and proliferation of cells. Conclusion: The cytokine expression profile from the cells seeded on DynaMatrix® suggests that it might be a suitable scaffold for regenerative endodontic procedures. It could improve vascularization by increasing angiogenic cytokines in the microenvironment of the treated root canal and supporting tissue regeneration.en_US
dc.identifier.urihttps://hdl.handle.net/1805/6494
dc.identifier.urihttp://dx.doi.org/10.7912/C2/1540
dc.language.isoen_USen_US
dc.subjectRegenerative Endodonticsen_US
dc.subjectEndodonticsen_US
dc.subjectCytokinesen_US
dc.subject.meshAngiogenic Proteinsen_US
dc.subject.meshCytokinesen_US
dc.subject.meshDental Pulp -- cytologyen_US
dc.subject.meshFibroblasts -- metabolismen_US
dc.subject.meshEndodontics -- methodsen_US
dc.subject.meshRegenerationen_US
dc.subject.meshTissue Engeineering -- methodsen_US
dc.subject.meshTissue Scaffoldsen_US
dc.titleEffects of DynaMatrix® on angiogenic cytokine expression from human dental pulp fibroblasts : an in vitro studyen_US
dc.typeThesisen
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Thesis of Joseph Benjamin Adams 2015.pdf
Size:
1.2 MB
Format:
Adobe Portable Document Format
Description:
Full Thesis
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.88 KB
Format:
Item-specific license agreed upon to submission
Description: