Adult Bone Marrow–derived Cells Do Not Acquire Functional Attributes of Cardiomyocytes When Transplanted into Peri-infarct Myocardium
dc.contributor.author | Scherschel, John A. | |
dc.contributor.author | Soonpaa, Mark H. | |
dc.contributor.author | Srour, Edward F. | |
dc.contributor.author | Field, Loren J. | |
dc.contributor.author | Rubart, Michael | |
dc.contributor.department | Microbiology and Immunology, School of Medicine | en_US |
dc.date.accessioned | 2020-12-09T16:22:36Z | |
dc.date.available | 2020-12-09T16:22:36Z | |
dc.date.issued | 2008-06-01 | |
dc.description.abstract | (BM) cells after being directly transplanted into the ischemically injured heart remains a controversial issue. In this study, we investigated the ability of transplanted BM cells to develop intracellular calcium ([Ca2+] i ) transients in response to membrane depolarization in situ. Low-density mononuclear (LDM) BM cells, c-kit-enriched (c-kitenr) BM cells, and highly enriched lin– c-kit+ BM cells were obtained from adult transgenic mice ubiquitously expressing enhanced green fluorescent protein (EGFP), and injected into peri-infarct myocardiums of nontransgenic mice. After 9–10 days the mice were killed, and the hearts were removed, perfused in Langendorff mode, loaded with the calcium-sensitive fluorophore rhod-2, and subjected to two-photon laser scanning fluorescence microscopy (TPLSM) to monitor action potential–induced [Ca2+] i transients in EGFP-expressing donor-derived cells and non-expressing host cardiomyocytes. Whereas spontaneous and electrically evoked [Ca2+] i transients were found to occur synchronously in host cardiomyocytes along the graft–host border and in areas remote from the infarct, they were absent in all of the >3,000 imaged BM-derived cells that were located in clusters throughout the infarct scar or peri-infarct zone. We conclude that engrafted BM-derived cells lack attributes of functioning cardiomyocytes, calling into question the concept that adult BM cells can give rise to substantive cardiomyocyte regeneration within the infarcted heart. | en_US |
dc.eprint.version | Final published version | en_US |
dc.identifier.citation | Scherschel, J. A., Soonpaa, M. H., Srour, E. F., Field, L. J., & Rubart, M. (2008). Adult bone marrow–derived cells do not acquire functional attributes of cardiomyocytes when transplanted into peri-infarct myocardium. Molecular Therapy, 16(6), 1129-1137. | en_US |
dc.identifier.uri | https://hdl.handle.net/1805/24556 | |
dc.language.iso | en_US | en_US |
dc.publisher | Elsevier | en_US |
dc.relation.isversionof | 10.1038/mt.2008.64 | en_US |
dc.relation.journal | The American Society of Gene Therapy | en_US |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.source | Publisher | en_US |
dc.subject | Bone Marrow | en_US |
dc.subject | Cells | en_US |
dc.subject | Functional Attributes | en_US |
dc.subject | Cardiomyocytes | en_US |
dc.subject | Peri-infarct Myocardium | en_US |
dc.title | Adult Bone Marrow–derived Cells Do Not Acquire Functional Attributes of Cardiomyocytes When Transplanted into Peri-infarct Myocardium | en_US |
dc.type | Article | en_US |