Subunit Association and DNA Binding Activity of the Heterotrimeric Transcription Factor NF-Y Is Regulated by Cellular Redox
dc.contributor.author | Nakshatri, Harikrishna | |
dc.contributor.author | Bhat-Nakshatri, Poornima | |
dc.contributor.author | Currie, R. Alexander | |
dc.date.accessioned | 2019-04-16T20:38:25Z | |
dc.date.available | 2019-04-16T20:38:25Z | |
dc.date.issued | 1996-11-15 | |
dc.description.abstract | NF-Y is a heterotrimeric transcription factor that specifically recognizes a CCAAT box motif found in a variety of eukaryotic promoter and enhancer elements. The subunit association and DNA binding properties of the NF-Y complex were examined as a function of redox state using recombinant NF-YA, NF-YB, and NF-YC subunits. Reduction of NF-YB by dithiothreitol (DTT) was essential for reconstitution of specific NF-Y CCAAT box DNA binding activity in vitro. Approximately 30% of the Escherichia coli-derived NF-YB subunit existed as intermolecular disulfide-linked dimers. NF-YB mutants in which the highly conserved cysteine residues at positions 85 and 89 had been converted to serines existed only as monomers and did not require DTT for functional NF-Y DNA binding activity. DTT was required, however, for the functional association of NF-YC with wild-type NF-YB but not with the NF-YB cysteine mutants. The cellular redox factors Ref-1 and adult T-cell leukemia-derived factor stimulated the DNA binding activity of recombinant NF-Y in the absence of DTT. Cells treated with 1-chloro-2,4-dinitrobenzene, an irreversible inhibitor of thioredoxin reductase, exhibited reduced endogenous NF-Y DNA binding activity. Together these results suggest that the cellular redox environment of mammalian cells is an important posttranscriptional regulator of NF-Y subunit association and DNA binding activities. | en_US |
dc.identifier.citation | Nakshatri, H., Bhat-Nakshatri, P., & Currie, R. A. (1996). Subunit Association and DNA Binding Activity of the Heterotrimeric Transcription Factor NF-Y Is Regulated by Cellular Redox. Journal of Biological Chemistry, 271(46), 28784–28791. https://doi.org/10.1074/jbc.271.46.28784 | en_US |
dc.identifier.doi | 10.1074/jbc.271.46.28784 | |
dc.identifier.issn | 0021-9258, 1083-351X | |
dc.identifier.uri | https://hdl.handle.net/1805/18866 | |
dc.language.iso | en_US | en_US |
dc.publisher | American Society for Biochemistry and Molecular Biology | en_US |
dc.subject | DNA | en_US |
dc.subject | NF-Y | en_US |
dc.subject | cellular redox | en_US |
dc.title | Subunit Association and DNA Binding Activity of the Heterotrimeric Transcription Factor NF-Y Is Regulated by Cellular Redox | en_US |
dc.type | Article | en_US |
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