Triptolide Directly Inhibits dCTP Pyrophosphatase
dc.contributor.author | Corson, Timothy W. | |
dc.contributor.author | Cavga, Hüseyin | |
dc.contributor.author | Aberle, Nicholas | |
dc.contributor.author | Crews, Craig M | |
dc.date.accessioned | 2013-08-20T16:55:57Z | |
dc.date.available | 2013-08-20T16:55:57Z | |
dc.date.issued | 2011-07 | |
dc.description.abstract | Triptolide is a potent natural product, with documented antiproliferative, immunosuppressive, anti-inflammatory, antifertility, and antipolycystic kidney disease effects. Despite a wealth of knowledge about the biology of this compound, direct intracellular target proteins have remained elusive. We synthesized a biotinylated photoaffinity derivative of triptolide, and used it to identify dCTP pyrophosphatase 1 (DCTPP1) as a triptolide-interacting protein. Free triptolide interacts directly with recombinant DCTPP1, and inhibits the enzymatic activity of this protein. Triptolide is thus the first dCTP pyrophosphatase inhibitor identified, and DCTPP1 is a biophysically validated target of triptolide. | en_US |
dc.identifier.citation | Corson, T. W., Cavga, H., Aberle, N., & Crews, C. M. (2011). Triptolide directly inhibits dCTP pyrophosphatase. ChemBioChem, 12(11), 1767-1773. | en_US |
dc.identifier.uri | https://hdl.handle.net/1805/3437 | |
dc.language.iso | en_US | en_US |
dc.subject | DCTPP1 | en_US |
dc.subject | natural products | en_US |
dc.subject | nucleotides | en_US |
dc.subject | photoaffinity labeling | en_US |
dc.subject | triptolide | en_US |
dc.title | Triptolide Directly Inhibits dCTP Pyrophosphatase | en_US |
dc.type | Article | en_US |