Browsing by Subject "voting mechanism"

Now showing 1 - 1 of 1
  • Thumbnail Image
    Item
    3D Centroidnet: Nuclei Centroid Detection with Vector Flow Voting
    (IEEE, 2022-10) Wu, Liming; Chen, Alain; Salama, Paul; Dunn, Kenneth W.; Delp, Edward J.; Electrical and Computer Engineering, School of Engineering and Technology
    Automated microscope systems are increasingly used to collect large-scale 3D image volumes of biological tissues. Since cell boundaries are seldom delineated in these images, detection of nuclei is a critical step for identifying and analyzing individual cells. Due to the large intra-class variability in nuclei morphology and the difficulty of generating ground truth annotations, accurate nuclei detection remains a challenging task. We propose a 3D nuclei centroid detection method by estimating the "vector flow" volume where each voxel represents a 3D vector pointing to its nearest nuclei centroid in the corresponding microscopy volume. We then use a voting mechanism to estimate the 3D nuclei centroids from the "vector flow" volume. Our system is trained on synthetic microscopy volumes and tested on real microscopy volumes. The evaluation results indicate our method outperforms other methods both visually and quantitatively.