Browsing by Subject "microradiography"

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    In vitro demineralization prevention by fluoride and silver nanoparticles when applied to sound enamel and enamel caries-like lesions of varying severities
    (Elsevier, 2021-01) Aldhaian, Bader A.; Balhaddad, Abdulrahman A.; Alfaifi, Areej A.; Levon, John A.; Eckert, George J.; Hara, Anderson T.; Lippert, Frank; Cariology, Operative Dentistry and Dental Public Health, School of Dentistry
    Objectives To investigate the effect of fluoride and silver nanoparticles on the prevention of in vitro demineralization of sound enamel and enamel caries-like lesions of varying severities. Methods Caries-like lesions of different severities (1/6/15 days) were created in bovine enamel specimens. One group remained sound. All specimens were demineralized again using a partially saturated acetic acid solution. Mimicking the intra-oral retention of fluoride and silver in vitro, this solution was supplemented with fluoride (0/1/10 ppm) and/or silver nanoparticles (0/10 ppm) in a factorial design. Changes in lesion depth (ΔL) and integrated mineral loss (ΔΔZ) were evaluated by digital transverse microradiography. Data was analyzed using three-way ANOVA. Results Lesion severity significantly affected ΔΔZ and ΔL, after no treatment and after the treatment of fluoride and silver independently (p = 0.012 and p = 0.037, respectively). Fluoride and the fluoride × lesion severity interaction were shown to be significant (p < 0.001) on ΔΔZ and ΔL. Silver nanoparticles significantly affected ΔΔZ (p = 0.041), but not ΔL (p = 0.15). The silver nanoparticles × lesion severity interaction was significant for ΔΔZ and ΔL (p = 0.032 and p = 0.024, respectively). No interaction was observed for ΔΔZ and ΔL between fluoride and silver (p = 0.962 and p = 0.971, respectively) as well as lesion severity and the use of fluoride and silver combined (p = 0.722 and p = 0.158, respectively). Conclusion Fluoride and silver nanoparticles had a significant effect on the prevention of in vitro demineralization of sound enamel and enamel caries-like lesions of varying severities. Clinical significance Fluoride and silver nanoparticles may potentially allow for more tailored caries prevention.
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    Indirect caries‐preventive effect of silver diamine fluoride on adjacent dental substrate: A single‐section demineralization study
    (Wiley, 2021-02) Romero, Maria Jacinta Rosario H.; Lippert, Frank; Cariology, Operative Dentistry and Dental Public Health, School of Dentistry
    This study assessed the indirect effect of 38% silver diamine fluoride (SDF) on demineralization of adjacent untreated sound and pre-demineralized enamel and dentine using a single-section model for digital transverse microradiography (TMR-D). Forty-eight bovine dentine single sections were demineralized, stratified (n = 12) according to integrated mineral loss (ΔZ), and treated with SDF or deionized water (DIW). Each “treated dentine” section was attached between untreated sound and pre-demineralized enamel or dentine and then subjected to demineralization. ΔZ and lesion depths (LD) of all specimens at baseline, 24 and 48 h demineralization, and after treatment of “treated dentine” were quantified using TMR-D. Fluoride in the demineralization solution of SDF clusters was determined using an ion-selective electrode. ΔZ and LD of sound and ΔZ of pre-demineralized enamel adjacent to SDF-treated dentine did not increase over time. All untreated dentine demineralized significantly; however, ΔZ of sound dentine adjacent to SDF-treated specimen was still significantly lower than control. SDF-treated dentine remineralized and released fluoride even after 48 h. Consistent with clinical findings, when applied only to demineralized teeth in this chemical model, 38% SDF completely inhibited demineralization in adjacent untreated sound enamel. Demineralization prevention was observed to a lesser extent in adjacent pre-demineralized enamel but not in dentine.