Browsing by Subject "mechanosensitive bone cells"

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    Vinculin Force Sensor Detects Tumor-Osteocyte Interactions
    (Springer Nature, 2019-04-04) Li, Fangjia; Chen, Andy; Reeser, Andrew; Wang, Yue; Fan, Yao; Liu, Shengzhi; Zhao, Xinyu; Prakash, Rahul; Kota, Divya; Li, Bai-Yan; Yokota, Hiroki; Liu, Jing; Physics, School of Science
    This study utilized a Förster resonance energy transfer (FRET)-based molecular tension sensor and live cell imaging to evaluate the effect of osteocytes, a mechanosensitive bone cell, on the migratory behavior of tumor cells. Two cell lines derived from MDA-MB-231 breast cancer cells were transfected with the vinculin tension sensor to quantitatively evaluate the force in focal adhesions of the tumor cell. Tumor cells treated with MLO-A5 osteocyte-conditioned media (CM) decreased the tensile forces in their focal adhesions and decreased their migratory potential. Tumor cells treated with media derived from MLO-A5 cells exposed to fluid flow-driven shear stress (FFCM) increased the tensile forces and increased migratory potential. Focal adhesion tension in tumor cells was also affected by distance from MLO-A5 cells when the two cells were co-cultured, where tumor cells close to MLO-A5 cells exhibited lower tension and decreased cell motility. Overall, this study demonstrates that focal adhesion tension is involved in altered migratory potential of tumor cells, and tumor-osteocyte interactions decrease the tension and motility of tumor cells.